| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
4-(1,2-Dihydroxyethyl)benzene-1,2-diol is a metabolite and does not have a direct pharmacological target. It is used as a biomarker for catecholamine turnover. It may have antioxidant properties due to its catechol structure. It may also interact with adrenergic receptors to a limited extent.
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| ln Vitro |
In vitro, 4-(1,2-Dihydroxyethyl)benzene-1,2-diol is used as a standard in analytical chemistry for the quantification of catecholamine metabolites. It can be measured in cell culture media, tissue extracts, and biological fluids using HPLC, LC-MS, or electrochemical detection. It is not typically used for biological activity assays.
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| ln Vivo |
In vivo, 4-(1,2-Dihydroxyethyl)benzene-1,2-diol is present in plasma and urine. Its concentration reflects catecholamine metabolism. It is used as a diagnostic and monitoring tool for conditions affecting catecholamine metabolism, such as pheochromocytoma and neuroblastoma.
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| Enzyme Assay |
For non-cellular assays, 4-(1,2-Dihydroxyethyl)benzene-1,2-diol is used as a standard for analytical method development and validation. It can be used in enzyme assays to measure the activity of MAO and other enzymes involved in catecholamine metabolism.
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| Cell Assay |
For in vitro cell-based assays, cells that produce catecholamines can be cultured, and 4-(1,2-Dihydroxyethyl)benzene-1,2-diol can be measured in the culture media as a marker of catecholamine metabolism. The effects of drugs or toxins on catecholamine metabolism can be assessed.
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| Animal Protocol |
For in vivo animal studies, 4-(1,2-Dihydroxyethyl)benzene-1,2-diol levels can be measured in plasma and urine to assess catecholamine metabolism. Animal models of pheochromocytoma, neuroblastoma, and other disorders affecting catecholamine metabolism can be studied.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties include solubility in water and organic solvents. It is a small molecule and is readily excreted in the urine. It is a metabolite and is not typically administered as a drug. Storage is typically at -20°C. It is a solid and should be handled with standard laboratory precautions.
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| Toxicity/Toxicokinetics |
Toxicological data for 4-(1,2-Dihydroxyethyl)benzene-1,2-diol is limited. As a naturally occurring metabolite, it is generally considered safe. No significant toxicity has been reported.
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| References | |
| Additional Infomation |
3,4-Dihydroxyphenylethylene glycol is a tetraol composed of an ethylene glycol molecule with a 3,4-dihydroxyphenyl group linked at the 1-position. It is a metabolite, specifically in mice. It belongs to the catechol family of compounds and is also a tetraol. 3,4-Dihydroxyphenylethylene glycol is a metabolite found or produced by Escherichia coli (strains K12 and MG1655). Reports indicate that 3,4-dihydroxyphenylethylene glycol has been detected in humans, olives, and beans, and relevant data are available.
4-(1,2-Dihydroxyethyl)benzene-1,2-diol is a metabolite of catecholamines. It is used as a biomarker for catecholamine metabolism. It is formed from norepinephrine and epinephrine by MAO and aldehyde reductase. It is excreted in the urine and can be measured for diagnostic purposes. |
| Molecular Formula |
C8H10O4
|
|---|---|
| Molecular Weight |
170.1626
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| Exact Mass |
170.057
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| CAS # |
28822-73-3
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| PubChem CID |
91528
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
453.2±40.0 °C at 760 mmHg
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| Melting Point |
128-129ºC
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| Flash Point |
236.6±21.9 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.665
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| LogP |
-1.3
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
12
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| Complexity |
139
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
MTVWFVDWRVYDOR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H10O4/c9-4-8(12)5-1-2-6(10)7(11)3-5/h1-3,8-12H,4H2
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| Chemical Name |
4-(1,2-dihydroxyethyl)benzene-1,2-diol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~587.68 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.69 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.69 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.69 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.8768 mL | 29.3841 mL | 58.7682 mL | |
| 5 mM | 1.1754 mL | 5.8768 mL | 11.7536 mL | |
| 10 mM | 0.5877 mL | 2.9384 mL | 5.8768 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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