| Size | Price | Stock | Qty |
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| ln Vitro |
Anti-proliferative activity against HSC-T6 cells (rat hepatic stellate cells):
HSC-T6 cells were treated with 3-O-Beta-D-Glucopyranosylplatycodigenin at concentrations of 10 μM, 25 μM, 50 μM, 75 μM, and 100 μM for 24 hours, 48 hours, and 72 hours. The compound exhibited concentration- and time-dependent anti-proliferative effects, with the most significant activity at 72 hours: - Proliferation inhibition rates: 12.3 ± 1.5% (10 μM), 28.5 ± 2.3% (25 μM), 45.8 ± 3.1% (50 μM), 59.2 ± 3.8% (75 μM), and 68.0 ± 4.2% (100 μM) [1] - The IC50 value for inhibiting HSC-T6 cell proliferation at 72 hours was 45.2 ± 3.6 μM [1] |
|---|---|
| Cell Assay |
HSC-T6 cell culture and anti-proliferative assay (MTT method):
1. Cell culture: HSC-T6 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin. Cells were maintained at 37°C in a 5% CO₂ humidified incubator and passaged every 2–3 days to keep in logarithmic growth [1] 2. Assay procedure: - Cells were seeded into 96-well plates at 5×10³ cells per well and incubated overnight to allow attachment [1] - The medium was replaced with fresh medium containing 3-O-Beta-D-Glucopyranosylplatycodigenin (final concentrations: 10 μM, 25 μM, 50 μM, 75 μM, 100 μM); the control group received medium without the compound [1] - After incubation for 24, 48, and 72 hours, 20 μL of MTT solution (5 mg/mL) was added to each well, followed by 4 hours of incubation at 37°C [1] - The supernatant was removed, and 150 μL of dimethyl sulfoxide (DMSO) was added to dissolve formazan crystals. Absorbance was measured at 570 nm using a microplate reader [1] - Proliferation inhibition rate was calculated as: (1 - Absorbance of sample group / Absorbance of control group) × 100%. IC50 was determined via nonlinear regression of the concentration-inhibition curve [1] |
| References | |
| Additional Infomation |
1. Background of 3-O-β-D-glucopyranosylplatinin: It is a novel oleanane-type triterpenoid compound isolated from the root of Platycodon grandiflorum. Platycodon grandiflorum is a traditional Chinese medicine that has historically been used to treat respiratory and digestive diseases[1]. 2. Significance of the study: Activation and proliferation of hepatic stellate cells (HSCs) are key drivers of liver fibrosis. The antiproliferative activity of 3-O-β-D-glucopyranosylplatinin against HSC-T6 cells suggests that it may have the potential to prevent or treat liver fibrosis, laying the foundation for further research on its in vivo efficacy and mechanism[1].
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| Molecular Formula |
C36H58O12
|
|---|---|
| Molecular Weight |
682.8385
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| Exact Mass |
682.393
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| CAS # |
38337-25-6
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| PubChem CID |
102185205
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| Appearance |
White to off-white solid powder
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| LogP |
0.944
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| Hydrogen Bond Donor Count |
9
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
48
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| Complexity |
1280
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| Defined Atom Stereocenter Count |
15
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| SMILES |
C[C@@]12CC[C@@H]3[C@@]([C@H]1CC=C4[C@]2(C[C@H]([C@@]5([C@H]4CC(CC5)(C)C)C(=O)O)O)C)(C[C@@H]([C@@H](C3(CO)CO)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O)O)C
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| InChi Key |
HPVWWHYNAAZHQR-KNSUCORESA-N
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| InChi Code |
InChI=1S/C36H58O12/c1-31(2)10-11-36(30(45)46)19(12-31)18-6-7-22-32(3)13-20(40)28(48-29-27(44)26(43)25(42)21(15-37)47-29)35(16-38,17-39)23(32)8-9-33(22,4)34(18,5)14-24(36)41/h6,19-29,37-44H,7-17H2,1-5H3,(H,45,46)/t19-,20-,21+,22+,23+,24+,25+,26-,27+,28-,29-,32+,33+,34+,36+/m0/s1
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| Chemical Name |
(4aR,5R,6aR,6aS,6bR,8aR,10R,11S,12aR,14bS)-5,11-dihydroxy-9,9-bis(hydroxymethyl)-2,2,6a,6b,12a-pentamethyl-10-[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-4a-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~146.45 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.66 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.66 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (3.66 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4645 mL | 7.3224 mL | 14.6447 mL | |
| 5 mM | 0.2929 mL | 1.4645 mL | 2.9289 mL | |
| 10 mM | 0.1464 mL | 0.7322 mL | 1.4645 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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