| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
3-Azidopropanol does not have a specific biological target itself but serves as a chemical reagent for bioconjugation and material functionalization. The azide group enables click chemistry reactions with alkyne-functionalized molecules through copper-catalyzed azide-alkyne cycloaddition (CuAAc) or strain-promoted azide-alkyne cycloaddition (SPAAC). The primary alcohol can be used for further functionalization or conjugation. This compound is widely used in the synthesis of drug delivery systems, biomaterials, and chemical biology probes.
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| ln Vitro |
The in vitro activity of 3-Azidopropanol is assessed by its ability to participate in click chemistry reactions. The efficiency of azide-alkyne cycloaddition is evaluated by HPLC or LC-MS analysis of the conjugation products. The compound's ability to form stable conjugates with alkyne-functionalized molecules is assessed by mass spectrometry or NMR spectroscopy. Reaction kinetics and biocompatibility are also evaluated in the presence of biological molecules or cells for bioconjugation applications.
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| ln Vivo |
In vivo studies for 3-Azidopropanol are typically conducted on the complete conjugates formed using this click chemistry reagent rather than the compound itself. These conjugates are evaluated in animal models for their pharmacokinetic properties, biodistribution, and therapeutic efficacy in applications such as drug delivery or imaging. The azide-alkyne click chemistry enables bioorthogonal labeling and conjugation without interfering with biological processes.
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| Enzyme Assay |
For click chemistry conjugation, 3-Azidopropanol is dissolved in appropriate solvent and reacted with alkyne-functionalized molecules in the presence of copper catalyst (CuAAc) or without catalyst (SPAAC with strained alkynes). The reaction is typically conducted at room temperature or 37°C for 2-24 hours. The extent of conjugation is monitored by HPLC, LC-MS, or TLC. The product is purified by column chromatography or preparative HPLC and characterized by NMR and mass spectrometry.
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| Cell Assay |
For cellular labeling studies, cells are first labeled with alkyne-containing probes and then treated with 3-Azidopropanol derivatives (e.g., fluorescent azide conjugates). Labeling efficiency is assessed by flow cytometry or fluorescence microscopy. For bioconjugation applications, proteins or other biomolecules are functionalized with alkynes and then conjugated to azide-containing molecules. The resulting conjugates are characterized by SDS-PAGE, mass spectrometry, or other appropriate methods.
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| Animal Protocol |
For in vivo imaging or therapeutic studies, mice bearing tumors or other targets are administered 3-Azidopropanol conjugates (e.g., fluorescent or drug conjugates) via intravenous injection. For imaging studies, fluorescence or radioactivity is monitored over time using appropriate imaging systems. For therapeutic studies, efficacy is evaluated by measuring tumor volume or disease biomarkers. Biodistribution is assessed by ex vivo analysis of organs and tissues at study termination.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 3-Azidopropanol are typically characterized for the complete conjugates rather than the click reagent itself. The compound has a molecular weight of 101.11 and is a small molecule with good solubility in water and organic solvents. The azide group is stable in biological environments while maintaining reactivity with alkynes. The compound is typically stored at 2-8°C or -20°C to maintain stability.
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| Toxicity/Toxicokinetics |
3-Azidopropanol is a chemical reagent intended for research use only and is not approved for human therapeutic use. As a click chemistry reagent, it is used in the synthesis of bioconjugates, drug delivery systems, and functional materials. The compound should be handled with standard laboratory safety precautions. The azide group can be potentially explosive, and appropriate safety measures should be taken when handling and storing this compound.
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| References | |
| Additional Infomation |
3-Azidopropanol is a click chemistry reagent with molecular formula C3H7N3O and molecular weight 101.11. The azide group enables copper-catalyzed or strain-promoted azide-alkyne cycloaddition with alkyne-functionalized molecules. The primary alcohol provides a handle for further functionalization. The compound is used in bioconjugation, drug delivery, and material science applications. It is for research use only and has not been approved for clinical applications.
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| Molecular Formula |
C3H7N3O
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|---|---|
| Molecular Weight |
101.11
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| Exact Mass |
101.059
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| CAS # |
72320-38-8
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| PubChem CID |
10996974
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| Appearance |
Colorless to light yellow liquid
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| Density |
1.095 g/cm3at 25°C (lit.)
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| Boiling Point |
64 °C at 2 Torr (lit.)
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| Flash Point |
87.77 °C - closed cup(lit.)
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| LogP |
0.131
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
7
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| Complexity |
76.2
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(CN=[N+]=[N-])CO
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| InChi Key |
WHVSIWLMCCGHFW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C3H7N3O/c4-6-5-2-1-3-7/h7H,1-3H2
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| Chemical Name |
3-azidopropan-1-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (989.02 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (24.73 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (24.73 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (24.73 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.8902 mL | 49.4511 mL | 98.9022 mL | |
| 5 mM | 1.9780 mL | 9.8902 mL | 19.7804 mL | |
| 10 mM | 0.9890 mL | 4.9451 mL | 9.8902 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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