| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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3',4'-Dimethoxyflavone is a natural lipophilic flavonoid tht can be isolated from the leaves of Primula veris, with various biological activities, including antioxidant, anti-cancer, anti-inflammatory, anti-atherogenic, hypolipidaemic, and neuroprotective or neurotrophic effects. It can reduce the synthesis and accumulation of PARP and protect cortical neurones against cell death induced by Parthanatos.
| ln Vitro |
3',4'-Dimethoxyflavone (10 and 20 μM) inhibits methylnitrosoguanidine (MNNG)-induced reduction in SH-SY5Y activity [2]. In HeLa cells, 3',4'-Dimethoxyflavone (6.25–25 μM) lowers MNNG-induced PAR levels [2]. When exposed to NMDA, 3',4'-dimethoxyflavone (12.5, 25, 50, and 100 μM; 15-20 hours) lessens cortical neuronal death [2]. Significant inhibition of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced EROD activity was seen with 3',4'-dimethoxyflavone (0.1-10 μM; 24 hours)[3]. In T47D and MCF-7 breast cancer cells, 3',4'-Dimethoxyflavone inhibits AhR-mediated inhibition of estrogen-induced gene expression as well as AhR-dependent CYP1A1 activation [3]. 3',4'-Dimethoxyflavone (2.5 μM; 7 days) stimulates the proliferation of human hematopoietic stem cells [4].
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| Cell Assay |
Cell Viability Assay[2]
Cell Types: Primary cortical neurons (isolated from fetal CD1 mice, incubated with NMDA) Tested Concentrations: 12.5, 25, 50 and 100 μM Incubation Duration: 15-20 hrs (hours) Experimental Results: Reduction by concentration Neuronal death caused by exposure to NMDA. Cell proliferation assay [4] Cell Types: CD34+ cells Tested Concentrations: 2.5 μM Incubation Duration: 7 days Experimental Results: Normoxic conditions induced Dramatically higher expansion of the CD34+ cell population. |
| References |
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| Additional Infomation |
3',4'-Dimethoxyflavone has been reported in Primula veris and Lawsonia inermis with data available.
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| Molecular Formula |
C17H14O4
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|---|---|
| Molecular Weight |
282.2907
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| Exact Mass |
282.089
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| CAS # |
4143-62-8
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| PubChem CID |
688674
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| Appearance |
White to light yellow solid powder
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| Density |
1.242g/cm3
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| Boiling Point |
428.5ºC at 760 mmHg
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| Melting Point |
154-155°C
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| Flash Point |
190.6ºC
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| Index of Refraction |
1.598
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| LogP |
3.477
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
21
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| Complexity |
417
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
ZGHORMOOTZTQFL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H14O4/c1-19-15-8-7-11(9-17(15)20-2)16-10-13(18)12-5-3-4-6-14(12)21-16/h3-10H,1-2H3
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| Chemical Name |
2-(3,4-dimethoxyphenyl)chromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 50 mg/mL (~177.12 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.86 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.86 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5425 mL | 17.7123 mL | 35.4246 mL | |
| 5 mM | 0.7085 mL | 3.5425 mL | 7.0849 mL | |
| 10 mM | 0.3542 mL | 1.7712 mL | 3.5425 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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