| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
(20S)-Protopanaxadiol targets raft-associated Akt signaling pathway[1]
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|---|---|
| ln Vitro |
In N2a cell rafts, 20S-protopanaxadiol treatment increased flotillin-1 levels to 142.91±10.71% of control. Depending on the type of cell, 20S-protopanaxadiol may have different effects on the raft-resident protein flotillin-1 [1].
(20S)-Protopanaxadiol regulates raft-associated Akt phosphorylation in a cell-type-specific manner; it enhances Akt Ser473 phosphorylation in HeLa and MCF-7 cells, while inhibiting it in PC12 and Neuro-2a cells[1] (20S)-Protopanaxadiol modulates the association of Akt with lipid rafts; it increases Akt recruitment to lipid rafts in HeLa/MCF-7 cells and reduces this recruitment in PC12/Neuro-2a cells, thereby altering downstream signaling (e.g., GSK-3β phosphorylation)[1] (20S)-Protopanaxadiol does not affect total Akt protein levels, only the raft-localized fraction and its phosphorylation status[1] |
| Cell Assay |
For lipid raft isolation and Akt association assay: Culture HeLa, MCF-7, PC12, and Neuro-2a cells in DMEM medium supplemented with fetal bovine serum; treat cells with (20S)-Protopanaxadiol (10 μM) for 24 hours; lyse cells with ice-cold lysis buffer containing detergent; separate lipid rafts via sucrose density gradient centrifugation; detect Akt and raft marker proteins (flotillin-1) in raft and non-raft fractions via Western blot[1]
For Akt phosphorylation assay: Culture target cells in DMEM medium; treat cells with (20S)-Protopanaxadiol (5-20 μM) for 12-48 hours; lyse cells and extract total protein; perform Western blot to detect phosphorylated Akt (Ser473), total Akt, and downstream target GSK-3β (phosphorylated and total forms); quantify band intensities via densitometry[1] For cell viability assay: Culture cells in 96-well plates; treat with (20S)-Protopanaxadiol (1-50 μM) for 48 hours; add MTT reagent and incubate for 4 hours; dissolve formazan crystals with DMSO; measure absorbance at 570 nm to evaluate cell viability[1] |
| References | |
| Additional Infomation |
(20S)-Protopanaxadiol is a diastereomer of protopanaxadiol in which a 20-hydroxy substituent is introduced into the pro-S position. It has been reported that (20S)-protopanaxadiol is found in Gynostemma pentaphyllum, ginseng and Acanthopanax senticosus, and there is relevant data. (20S)-Protopanaxadiol is the main metabolite of ginsenosides, which are a class of active compounds isolated from ginseng [1]. The cell type-specific regulation of lipid raft-related Akt signaling pathway by (20S)-protopanaxadiol is attributed to the differences in lipid raft composition and downstream signaling networks among different cell types [1]. (20S)-Protopanaxadiol may exert cell type-specific biological effects (e.g., by promoting cancer cell proliferation through regulation of raft-dependent Akt signaling, while protecting neurons [1]).
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| Molecular Formula |
C30H52O3
|
|---|---|
| Molecular Weight |
460.73208
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| Exact Mass |
460.391
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| CAS # |
30636-90-9
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| PubChem CID |
11213350
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
559.5±40.0 °C at 760 mmHg
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| Melting Point |
211 °C
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| Flash Point |
226.1±21.9 °C
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| Vapour Pressure |
0.0±3.5 mmHg at 25°C
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| Index of Refraction |
1.529
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| LogP |
7.59
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
33
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| Complexity |
783
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| Defined Atom Stereocenter Count |
10
|
| SMILES |
CC(=CCC[C@@](C)([C@H]1CC[C@@]2([C@@H]1[C@@H](C[C@H]3[C@]2(CC[C@@H]4[C@@]3(CC[C@@H](C4(C)C)O)C)C)O)C)O)C
|
| InChi Key |
PYXFVCFISTUSOO-HKUCOEKDSA-N
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| InChi Code |
InChI=1S/C30H52O3/c1-19(2)10-9-14-30(8,33)20-11-16-29(7)25(20)21(31)18-23-27(5)15-13-24(32)26(3,4)22(27)12-17-28(23,29)6/h10,20-25,31-33H,9,11-18H2,1-8H3/t20-,21+,22-,23+,24-,25-,27-,28+,29+,30-/m0/s1
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| Chemical Name |
(3S,5R,8R,9R,10R,12R,13R,14R,17S)-17-[(2S)-2-hydroxy-6-methylhept-5-en-2-yl]-4,4,8,10,14-pentamethyl-2,3,5,6,7,9,11,12,13,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthrene-3,12-diol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~217.05 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1705 mL | 10.8523 mL | 21.7047 mL | |
| 5 mM | 0.4341 mL | 2.1705 mL | 4.3409 mL | |
| 10 mM | 0.2170 mL | 1.0852 mL | 2.1705 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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