| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
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| Other Sizes |
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| ln Vitro |
While addition of L-ascorbic acid (Asc) only marginally enhanced cell growth, trisodium L-ascorbic acid 2-phosphate (0.1-1.5 mM; media changes every 2 to 3 days for 2 to 3 weeks) greatly stimulated cell growth. stimulation of growth. Cell growth is greatly enhanced by the combination of Asc-2P and bFGF; however, additional effects are not produced by the addition of EGF or insulin [1]. Higher doses of AsA2-P lead to enhanced runx2 expression and ALP activity. L-ascorbic acid 2-phosphate (50 µM-250 µM) trisodium is required for the efficient osteogenic differentiation of human adipose stem cells (hASC). The combinations of 150 µM asA2-P and 10 nM dexamethasone (Dex) and 250 µM asA2-P and 5 nM Dex produced the maximum proliferation, ALP activity, and runx2 expression [3].
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|---|---|
| References |
|
| Molecular Formula |
C6H6NA3O9P
|
|---|---|
| Molecular Weight |
322.05
|
| Exact Mass |
321.944
|
| CAS # |
66170-10-3
|
| Related CAS # |
L-Ascorbic acid 2-phosphate magnesium;113170-55-1;L-Ascorbic acid 2-phosphate;23313-12-4
|
| PubChem CID |
10990876
|
| Appearance |
White to off-white solid powder
|
| Melting Point |
84 °C
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
3
|
| Heavy Atom Count |
19
|
| Complexity |
357
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
C([C@@H]([C@@H]1C(=C(C(=O)O1)OP(=O)([O-])[O-])[O-])O)O.[Na+].[Na+].[Na+]
|
| InChi Key |
YRWWOAFMPXPHEJ-OFBPEYICSA-K
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| InChi Code |
InChI=1S/C6H9O9P.3Na/c7-1-2(8)4-3(9)5(6(10)14-4)15-16(11,12)13;;;/h2,4,7-9H,1H2,(H2,11,12,13);;;/q;3*+1/p-3/t2-,4+;;;/m0.../s1
|
| Chemical Name |
sodium (R)-5-((S)-1,2-dihydroxyethyl)-2-oxido-4-oxo-4,5-dihydrofuran-3-yl phosphate
|
| Synonyms |
2-Phospho-L-ascorbic acid trisodium salt VOK-70103 VOK70103VOK 70103523114
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| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~150 mg/mL (~465.77 mM)
DMSO : ~6 mg/mL (~18.63 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 120 mg/mL (372.61 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1051 mL | 15.5255 mL | 31.0511 mL | |
| 5 mM | 0.6210 mL | 3.1051 mL | 6.2102 mL | |
| 10 mM | 0.3105 mL | 1.5526 mL | 3.1051 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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