| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
|
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| Other Sizes |
| ln Vitro |
- Rat isolated ileum preparation: 2-Hydroxy-6-methoxybenzoic acid exerts spasmolytic activity by inhibiting acetylcholine-induced and histamine-induced ileum smooth muscle contraction in a concentration-dependent manner[2]
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|---|---|
| ln Vivo |
- Mouse analgesic assay (hot plate test): Intraperitoneal administration of 2-Hydroxy-6-methoxybenzoic acid (50, 100 mg/kg) significantly increases the latency period of pain response compared to the control group[2]
- Mouse analgesic assay (acetic acid-induced writhing test): 2-Hydroxy-6-methoxybenzoic acid (50, 100 mg/kg, i.p.) reduces the number of writhing responses induced by acetic acid in a dose-dependent manner[2] - Rat intestinal spasm model: 2-Hydroxy-6-methoxybenzoic acid (100 mg/kg, i.p.) inhibits barium chloride-induced intestinal spasm, reducing the frequency and amplitude of intestinal contractions[2] |
| Animal Protocol |
- Analgesic activity test (hot plate test): Female mice are acclimated to the hot plate apparatus (55±0.5℃) to determine baseline latency. Mice are randomly divided into control group (vehicle) and 2-Hydroxy-6-methoxybenzoic acid groups (50, 100 mg/kg). The test compound is administered intraperitoneally, and pain latency is measured at 30, 60, 90, 120 minutes after administration. A cut-off time of 30 seconds is set to avoid tissue damage[2]
- Analgesic activity test (acetic acid-induced writhing test): Male mice are divided into control and treatment groups. 2-Hydroxy-6-methoxybenzoic acid (50, 100 mg/kg) is administered intraperitoneally 30 minutes before intraperitoneal injection of acetic acid (0.6%). The number of writhing responses (abdominal constriction, hind limb extension) is counted within 15 minutes after acetic acid injection[2] - Spasmolytic activity test in rats: Male rats are sacrificed, and the ileum segment (2-3 cm) is isolated and mounted in a tissue bath containing Krebs-Ringer bicarbonate buffer (37℃, aerated with 95% O₂ and 5% CO₂). After equilibration, the ileum is pre-treated with 2-Hydroxy-6-methoxybenzoic acid (10, 30, 100 μM) for 10 minutes, followed by addition of acetylcholine or histamine to induce contraction. The contractile response is recorded via a force transducer, and the inhibition rate is calculated compared to the control[2] |
| References |
|
| Additional Infomation |
6-Methoxysalicylic acid is a methoxybenzoic acid. 2-Hydroxy-6-methoxybenzoic acid has been reported to be found in Colchicum manissadjianii, Colchicum kurdicum, and other organisms with relevant data. 2-Hydroxy-6-methoxybenzoic acid is a bioactive compound isolated from the plant Brickellia veronicifolia [2]. The antispasmodic and analgesic activities of 2-Hydroxy-6-methoxybenzoic acid contribute to the use of Brickellia veronicifolia in traditional medicine for the treatment of gastrointestinal spasms and pain [2].
|
| Molecular Formula |
C8H8O4
|
|---|---|
| Molecular Weight |
168.14672
|
| Exact Mass |
168.042
|
| CAS # |
3147-64-6
|
| PubChem CID |
591524
|
| Appearance |
White to off-white solid powder
|
| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
330.4±27.0 °C at 760 mmHg
|
| Melting Point |
134-138
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| Flash Point |
138.7±17.2 °C
|
| Vapour Pressure |
0.0±0.8 mmHg at 25°C
|
| Index of Refraction |
1.586
|
| LogP |
0.9
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
12
|
| Complexity |
168
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
COC1=CC=CC(O)=C1C(O)=O
|
| InChi Key |
AAUQLHHARJUJEH-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C8H8O4/c1-12-6-4-2-3-5(9)7(6)8(10)11/h2-4,9H,1H3,(H,10,11)
|
| Chemical Name |
2-hydroxy-6-methoxybenzoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~594.71 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.87 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.87 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.87 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9471 mL | 29.7354 mL | 59.4707 mL | |
| 5 mM | 1.1894 mL | 5.9471 mL | 11.8941 mL | |
| 10 mM | 0.5947 mL | 2.9735 mL | 5.9471 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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