| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
2-Di-1-ASP targets G-quadruplex (G4) DNA structures and double-stranded DNA. G-quadruplexes are four-stranded DNA structures formed by guanine-rich sequences that play important roles in gene regulation, telomere maintenance, and genome stability. By selectively binding to G-quadruplex structures, 2-Di-1-ASP serves as a fluorescent probe for detecting and studying these structures. It also binds to the groove of double-stranded DNA as a fluorescent probe.
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|---|---|
| ln Vitro |
2-Di-1-ASP (compound 18a) has good selectivity for double-stranded DNA and shows a notable fluorescence enhancement (up to 300-fold) in the presence of G-quadruplex (G4) structure. For parallel G4-DNA forms (c-kit2, c-kit87up, and c-myc), 2-Di-1-ASP exhibits fluorescence selectivity [1].
In vitro, 2-Di-1-ASP functions as a fluorescent probe for DNA structures. It shows fluorimetric selectivity for parallel G4-DNA forms including c-kit2, c-kit87up, and c-myc. The compound demonstrates notable fluorescence enhancement (up to 300-fold) in the presence of G-quadruplex structures. It is widely used as a mitochondrial stain and groove-binding fluorescent probe for double-stranded DNA. Its fluorescence properties are characterized by spectrophotometric and fluorometric methods. |
| ln Vivo |
In vivo, 2-Di-1-ASP is used as a fluorescent probe for staining mitochondria and DNA in cells and tissues. Its ability to selectively bind to G-quadruplex structures and double-stranded DNA makes it useful for studying DNA structure and function in live cells. However, specific in vivo applications are primarily focused on cellular imaging and staining rather than therapeutic applications. The compound is a research tool for cell biology and DNA structure studies.
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| Enzyme Assay |
Cell-free assays for 2-Di-1-ASP involve characterizing its fluorescence properties and DNA binding affinity. The compound is incubated with various DNA structures (G-quadruplex, double-stranded DNA, single-stranded DNA) in buffered solutions. Fluorescence enhancement is measured using a fluorometer. Binding selectivity is assessed by comparing fluorescence intensity in the presence of different DNA structures. Binding affinity is determined from fluorescence titration experiments. Chemical purity and identity are confirmed by HPLC and NMR analysis.
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| Cell Assay |
In vitro cellular assays for 2-Di-1-ASP typically involve staining cells with the compound to visualize mitochondria and DNA. Cells are incubated with 2-Di-1-ASP at appropriate concentrations for defined periods. Fluorescence is visualized by fluorescence microscopy or quantified by flow cytometry. The compound's ability to stain mitochondria and DNA is assessed. Co-localization studies with other organelle-specific dyes are performed to confirm specificity. Cytotoxicity is evaluated to ensure that the compound is well-tolerated at staining concentrations.
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| Animal Protocol |
In vivo animal studies for 2-Di-1-ASP are limited, as the compound is primarily used as a fluorescent probe for in vitro and ex vivo applications. The compound may be used for tissue staining or for studying DNA structures in animal models. However, specific in vivo applications are not extensively documented in the available literature. The compound is intended for research use only as a fluorescent probe.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 2-Di-1-ASP include a molecular weight of 366.24 g/mol and molecular formula C16H19IN2. The compound has a purity of ≥99%. As a fluorescent dye, its pharmacokinetic properties are not the primary focus of study. The compound is typically stored at appropriate conditions as a research reagent. Detailed ADME parameters are not extensively reported in the available literature.
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| Toxicity/Toxicokinetics |
The toxicity profile of 2-Di-1-ASP has not been extensively characterized in published literature. As a fluorescent probe, it is typically used at low concentrations for staining applications. Standard safety precautions should be followed when handling this compound. It is intended for research use only as a fluorescent probe, not for therapeutic applications.
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| References | |
| Additional Infomation |
2-[4-(dimethylamino)styryl]-1-methylpyridine iodide is a pyridine salt, tertiary amine, and organic iodide salt. It is used as a fluorescent dye. It contains 2-[4-(dimethylamino)styryl]-1-methylpyridinium.
2-Di-1-ASP (DASPI) is a mono-styryl dye widely used as a mitochondrial stain and groove-binding fluorescent probe for double-stranded DNA. It shows selectivity for G-quadruplex (G4) and double-stranded DNA and demonstrates up to 300-fold fluorescence enhancement in the presence of G4 structures. 2-Di-1-ASP is a research tool for studying DNA structure, mitochondria, and cellular imaging. |
| Molecular Formula |
C16H19N2+.I-
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|---|---|
| Molecular Weight |
366.23946
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| Exact Mass |
366.059
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| CAS # |
2156-29-8
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| PubChem CID |
5463010
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| Appearance |
Pink to red solid powder
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| Density |
1.091g/cm3
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| Boiling Point |
397.8℃at 760 mmHg
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| Melting Point |
280ºC (dec.)(lit.)
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| Flash Point |
179.3℃
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| Vapour Pressure |
1.55E-06mmHg at 25°C
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| Index of Refraction |
1.668
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
19
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| Complexity |
264
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C[N+]1=CC=CC=C1/C=C/C2=CC=C(C=C2)N(C)C.[I-]
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| InChi Key |
XPOIQAIBZGSIDD-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C16H19N2.HI/c1-17(2)15-10-7-14(8-11-15)9-12-16-6-4-5-13-18(16)3;/h4-13H,1-3H3;1H/q+1;/p-1
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| Chemical Name |
N,N-dimethyl-4-[(E)-2-(1-methylpyridin-1-ium-2-yl)ethenyl]aniline;iodide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~68.26 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.68 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.68 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7304 mL | 13.6522 mL | 27.3045 mL | |
| 5 mM | 0.5461 mL | 2.7304 mL | 5.4609 mL | |
| 10 mM | 0.2730 mL | 1.3652 mL | 2.7304 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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