| Size | Price | Stock | Qty |
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| 1g |
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| 2g |
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| 5g |
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| 10g |
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| 25g |
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| Other Sizes |
Purity: ≥98%
| Targets |
2′-Deoxycytidine hydrochloride does not have a specific pharmacological target. As a nucleoside, it is incorporated into DNA during replication and is a substrate for enzymes involved in nucleotide metabolism, including deoxycytidine kinase and cytidine deaminase. It is an essential component of DNA synthesis and is used in cell culture to support cell growth and proliferation.
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| ln Vitro |
In vitro, 2′-Deoxycytidine hydrochloride is used as a nucleoside supplement in cell culture media to support DNA synthesis and cell proliferation. It is a substrate for deoxycytidine kinase, which phosphorylates it to deoxycytidine monophosphate (dCMP), a precursor for DNA synthesis. It is also used in studies of nucleotide metabolism and DNA replication.
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| ln Vivo |
In vivo, 2′-Deoxycytidine hydrochloride is a naturally occurring nucleoside that is involved in DNA synthesis. It is not a therapeutic agent but is used as a nutritional supplement in cell culture and research. It is sometimes used in combination with other nucleosides to support cell growth in serum-free media.
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| Enzyme Assay |
In vitro enzyme assays for 2′-Deoxycytidine hydrochloride involve measuring the activity of enzymes that metabolize it, such as deoxycytidine kinase or cytidine deaminase. The enzyme is incubated with the substrate and cofactors, and the reaction product is measured by HPLC or spectrophotometry. The compound's stability in various media can also be assessed.
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| Cell Assay |
In vitro cell-based assays for 2′-Deoxycytidine hydrochloride are performed to assess its effects on cell growth and proliferation. Cells are cultured in media with or without 2′-Deoxycytidine supplementation, and cell proliferation is measured by cell counting or MTT assays. The compound's effects on nucleotide pools and DNA synthesis can be assessed by labeling with radioactive or fluorescent nucleotides.
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| Animal Protocol |
In vivo animal studies for 2′-Deoxycytidine hydrochloride are not typical, as it is a naturally occurring nucleoside and a nutrient supplement. If conducted, they would assess its effects on nucleotide metabolism and DNA synthesis in various tissues. However, specific protocols are not widely available.
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| ADME/Pharmacokinetics |
2′-Deoxycytidine hydrochloride is absorbed from the gastrointestinal tract and is distributed to various tissues. It is phosphorylated to deoxycytidine monophosphate (dCMP) by deoxycytidine kinase and incorporated into DNA. It is metabolized by cytidine deaminase to deoxyuridine. It is excreted primarily in urine.
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| Toxicity/Toxicokinetics |
2′-Deoxycytidine hydrochloride is generally recognized as safe as a nutritional supplement and cell culture reagent. It is not associated with significant toxicity at concentrations used in cell culture. High doses may cause effects related to nucleotide metabolism, but it is not classified as a carcinogen or mutagen.
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| References | |
| Additional Infomation |
2′-Deoxycytidine hydrochloride is a nucleoside analog used as a precursor in DNA synthesis. It is used in cell culture media as a nucleoside supplement to support cell growth and proliferation. It is a substrate for enzymes involved in nucleotide metabolism. This product is for research and cell culture use.
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| Molecular Formula |
C9H14CLN3O4
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|---|---|
| Molecular Weight |
263.6782
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| Exact Mass |
263.067
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| CAS # |
3992-42-5
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| Related CAS # |
2'-Deoxycytidine;951-77-9
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| PubChem CID |
107488
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| Appearance |
White to off-white solid powder
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| Melting Point |
168-170°C
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| Flash Point |
254.8ºC
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| Index of Refraction |
57 ° (C=1, H2O)
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
17
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| Complexity |
355
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1[C@@H]([C@H](O[C@H]1N2C=CC(=NC2=O)N)CO)O.Cl
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| InChi Key |
LTKCXZGFJFAPLY-OERIEOFYSA-N
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| InChi Code |
InChI=1S/C9H13N3O4.ClH/c10-7-1-2-12(9(15)11-7)8-3-5(14)6(4-13)16-8;/h1-2,5-6,8,13-14H,3-4H2,(H2,10,11,15);1H/t5-,6+,8+;/m0./s1
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| Chemical Name |
4-amino-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one;hydrochloride
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| Synonyms |
2'-Deoxycytidine hydrochloride; 2'-Deoxycytidine monohydrochloride; Deoxycytidine hydrochloride; NSC83251; NSC 83251; NSC-83251;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 41 mg/mL (~155.49 mM)
DMSO : ~25 mg/mL (~94.81 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (9.48 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (9.48 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 50 mg/mL (189.62 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7925 mL | 18.9624 mL | 37.9248 mL | |
| 5 mM | 0.7585 mL | 3.7925 mL | 7.5850 mL | |
| 10 mM | 0.3792 mL | 1.8962 mL | 3.7925 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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