| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
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| Other Sizes |
| Targets |
2'-Deoxyadenosine monohydrate is an endogenous metabolite and a DNA nucleoside. It serves as a substrate for various enzymes involved in DNA synthesis and repair, including DNA polymerases and kinases. It can also affect cellular cAMP levels and may be used by some cells as an energy source under conditions of energy stress.
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|---|---|
| ln Vitro |
In vitro, 2'-Deoxyadenosine monohydrate is used as a building block or intermediate in chemical synthesis. It is a substrate for kinases that phosphorylate it to deoxyadenosine monophosphate (dAMP), a precursor for DNA synthesis. In cell culture, it can be added to media to support cell growth and proliferation. Its biological activity is primarily related to its role in nucleotide metabolism.
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| ln Vivo |
In vivo, 2'-Deoxyadenosine monohydrate is a normal constituent of the body's nucleotide pool. It is incorporated into DNA during replication and repair processes. It does not have a direct pharmacological activity but is essential for life. Deficiencies or imbalances in nucleotide pools can lead to various health issues.
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| Enzyme Assay |
Enzymatic assays can be performed to measure the activity of kinases that phosphorylate 2'-Deoxyadenosine. For example, deoxyadenosine kinase can be incubated with 2'-Deoxyadenosine and ATP, and the production of dAMP is measured using HPLC or a coupled enzyme assay. These assays are used to characterize enzyme kinetics and screen for inhibitors.
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| Cell Assay |
Cellular assays with 2'-Deoxyadenosine monohydrate are typically conducted to study nucleotide metabolism and DNA synthesis. Cells are cultured with radiolabeled or otherwise modified 2'-Deoxyadenosine, and its incorporation into DNA is measured. It can also be used to study the effects of nucleoside analogs on cell proliferation and viability.
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| Animal Protocol |
2'-Deoxyadenosine monohydrate is not typically administered as a drug in animal studies. However, it can be used in studies of nucleotide metabolism. For instance, it might be administered to animals to trace metabolic pathways or to study the effects of nucleoside analogs on DNA synthesis and repair.
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| ADME/Pharmacokinetics |
2'-Deoxyadenosine monohydrate has a molecular weight of 269.26 g/mol and a chemical formula of C10H15N5O4. As a naturally occurring nucleoside, it is water-soluble and is efficiently absorbed and metabolized by the body. It is stable as a solid and is typically stored at room temperature.
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| Toxicity/Toxicokinetics |
2'-Deoxyadenosine monohydrate is an endogenous, naturally occurring compound and is generally considered safe. It is a normal metabolite and is not associated with toxicity at physiological concentrations. It is suitable for cell culture applications.
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| Additional Infomation |
2'-Deoxyadenosine monohydrate is a standard nucleoside used extensively in biochemical and molecular biology research. It is employed as a building block for the synthesis of DNA, nucleoside analogs, and other bioactive molecules. It is often used in comparison studies of the functions of adenosine analogues on various biological processes.
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| Molecular Formula |
C10H15N5O4
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|---|---|
| Molecular Weight |
269.2572
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| Exact Mass |
269.112
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| CAS # |
16373-93-6
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| PubChem CID |
9549172
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| Appearance |
White to off-white solid powder
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| Density |
1.9±0.1 g/cm3
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| Boiling Point |
627.2±65.0 °C at 760 mmHg
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| Melting Point |
185-187ºC
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| Flash Point |
333.1±34.3 °C
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| Vapour Pressure |
0.0±1.9 mmHg at 25°C
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| Index of Refraction |
1.863
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| LogP |
-0.55
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
19
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| Complexity |
307
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1[C@@H]([C@H](O[C@H]1N2C=NC3=C(N=CN=C32)N)CO)O.O
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| InChi Key |
WZJWHIMNXWKNTO-VWZUFWLJSA-N
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| InChi Code |
InChI=1S/C10H13N5O3.H2O/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(17)6(2-16)18-7;/h3-7,16-17H,1-2H2,(H2,11,12,13);1H2/t5-,6+,7+;/m0./s1
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| Chemical Name |
(2R,3S,5R)-5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-ol;hydrate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~185.69 mM)
H2O : ~7.14 mg/mL (~26.52 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.28 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7139 mL | 18.5694 mL | 37.1388 mL | |
| 5 mM | 0.7428 mL | 3.7139 mL | 7.4278 mL | |
| 10 mM | 0.3714 mL | 1.8569 mL | 3.7139 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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