| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
(2-Chloropyridin-4-yl)methanamine hydrochloride targets lysyl oxidase-like 2 (LOXL2), an enzyme that catalyzes the cross-linking of collagen and elastin. It is a selective inhibitor with an IC50 of 126 nM. By inhibiting LOXL2, it may modulate extracellular matrix remodeling and fibrosis.
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| ln Vitro |
Selectivity for LOXL2 and other hydroxylamine oxidases (MAO-A, MAO-B, and SSAO) of 2-chloropyridin-4-yl)methylamine hydrochloride derivative. The IC50 of 2-chloropyridine)-4-yl)methanamine hydrochloride in the human whole blood LOXL2 assay was 1.45 μM and 126 nM in the absence of saline protein. The selectivity of 2-chloropyridin-4-yl) methylamine hydrochloride for LOXL2+BSA (IC50=190 nM) is 31 times higher than that of LOX+BSA (IC50=5.91μM). When tested at 30 μM against a panel of strong LTQ AO enzymes (MAO-A, MAO-B, and SSAO), LOXL2-IN-1 was found to be inactive. Different CYP enzymes (CYP 3A4, 2C9, and 2D6) can be inhibited by 2-chloropyridin-4-yl methylamine hydrochloride, with a maximal IC50 surpassing 30 μM [1].
In vitro, (2-Chloropyridin-4-yl)methanamine hydrochloride demonstrates selective LOXL2 inhibition with an IC50 of 126 nM. It is a chemical intermediate used to build drugs or small-molecule ligands. |
| ln Vivo |
In vivo, (2-Chloropyridin-4-yl)methanamine hydrochloride is not used as a therapeutic agent. It is a chemical intermediate and research tool for studying LOXL2 inhibition.
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| Enzyme Assay |
The in vitro LOXL2 enzyme assay involves incubating the enzyme with a substrate in the presence of varying concentrations of the compound. The enzyme activity is measured, and the IC50 is calculated from the inhibition curve.
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| Cell Assay |
In vitro cell-based assays for (2-Chloropyridin-4-yl)methanamine hydrochloride are not typical, as it is primarily a chemical intermediate. Its LOXL2 inhibitory activity could be studied in cells expressing LOXL2.
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| ADME/Pharmacokinetics |
(2-Chloropyridin-4-yl)methanamine hydrochloride has a molecular weight of 179.05 g/mol and is soluble in DMSO. It is typically stored as a powder at -20°C. Its purity is ≥95%.
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| Toxicity/Toxicokinetics |
Toxicological data for (2-Chloropyridin-4-yl)methanamine hydrochloride are limited, as it is a research compound. It is not intended for human use. Standard safety precautions for handling chemical reagents should be followed.
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| References | |
| Additional Infomation |
(2-Chloropyridin-4-yl)methanamine hydrochloride is a research compound with no clinical approval. It is a valuable tool for studying LOXL2 biology and the role of LOXL2 in fibrosis and cancer. It is also used as a chemical intermediate in organic synthesis.
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| Molecular Formula |
C6H8CL2N2
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|---|---|
| Molecular Weight |
179.0471
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| Exact Mass |
178.006
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| CAS # |
916210-98-5
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| PubChem CID |
66570668
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
2.696
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
10
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| Complexity |
87.1
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
BWSUKLOBJGMGCJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H7ClN2.ClH/c7-6-3-5(4-8)1-2-9-6;/h1-3H,4,8H2;1H
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| Chemical Name |
(2-chloropyridin-4-yl)methanamine;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 50 mg/mL (~279.25 mM)
DMSO : ≥ 33 mg/mL (~184.31 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (558.50 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.5850 mL | 27.9252 mL | 55.8503 mL | |
| 5 mM | 1.1170 mL | 5.5850 mL | 11.1701 mL | |
| 10 mM | 0.5585 mL | 2.7925 mL | 5.5850 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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