| Size | Price | Stock | Qty |
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| 10g |
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| Other Sizes |
| Targets |
2-Aminobenzenesulfonamide targets carbonic anhydrase IX (CA IX) (Ki = 450 nM) [1]
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|---|---|
| ln Vitro |
- 2-Aminobenzenesulfonamide exhibited selective inhibitory activity against the tumor-associated CA IX isozyme, with a Ki value of 450 nM [1]
- The compound showed weaker inhibitory effects on other CA isozymes (e.g., CA I, CA II) compared to CA IX, indicating isozyme selectivity [1] - In enzyme inhibition assays, 2-Aminobenzenesulfonamide dose-dependently suppressed CA IX catalytic activity, achieving 50% inhibition at concentrations corresponding to its Ki value [1] |
| Enzyme Assay |
- Recombinant human CA IX enzyme was used for the inhibition assay; the reaction was performed in a buffer system at a specific pH [1]
- p-Nitrophenyl acetate was used as the substrate, and the hydrolysis of the substrate by CA IX was monitored spectrophotometrically by measuring the increase in absorbance at a specific wavelength [1] - Serial dilutions of 2-Aminobenzenesulfonamide were added to the reaction mixture, and the rate of substrate hydrolysis was recorded for each concentration [1] - The inhibition constant (Ki) was calculated using nonlinear regression analysis based on the dose-response curves of enzyme activity inhibition [1] |
| References | |
| Additional Infomation |
2-Aminobenzenesulfonamide is a sulfonamide compound belonging to the benzene class. - 2-Aminobenzenesulfonamide is an aromatic sulfonamide carbonic anhydrase inhibitor [1] - The inhibitory activity of this compound against CA IX is attributed to its ability to bind to the active site of the enzyme, thereby competing with the natural substrate (carbon dioxide) [1] - CA IX is overexpressed in a variety of tumors and plays a key role in tumor acidification and progression, making it a potential target for anticancer therapy [1].
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| Molecular Formula |
C6H8N2O2S
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|---|---|
| Molecular Weight |
172.20492
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| Exact Mass |
172.03
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| CAS # |
3306-62-5
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| PubChem CID |
72894
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| Appearance |
White to light brown solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
392.7±44.0 °C at 760 mmHg
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| Melting Point |
155-157 °C(lit.)
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| Flash Point |
191.3±28.4 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.628
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| LogP |
0.22
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
11
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| Complexity |
219
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
YAZSBRQTAHVVGE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H8N2O2S/c7-5-3-1-2-4-6(5)11(8,9)10/h1-4H,7H2,(H2,8,9,10)
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| Chemical Name |
2-aminobenzenesulfonamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~200 mg/mL (~1161.44 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (29.04 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (29.04 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.8072 mL | 29.0360 mL | 58.0720 mL | |
| 5 mM | 1.1614 mL | 5.8072 mL | 11.6144 mL | |
| 10 mM | 0.5807 mL | 2.9036 mL | 5.8072 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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