| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
2-Amino-5-phenylpyridine does not have a specific therapeutic target. As a mutagenic heterocyclic amine, it is known to induce mutations at the molecular level, potentially leading to various cellular effects. Its primary relevance is in toxicology and food safety as a potential carcinogen.
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|---|---|
| ln Vitro |
However, 5-phenyl-2-pyridylamine (PPA) shares a lot of structural similarities with aminobiphenyls, a different class of well-known aromatic amines that cause cancer [2].
In vitro, 2-amino-5-phenylpyridine is used as a model mutagenic compound. Its mutagenic properties can be assessed using the Ames test (Salmonella typhimurium assay). It can also be used to study the mechanisms of DNA adduct formation and mutagenesis. It serves as a key synthetic intermediate for constructing bioactive molecules. |
| ln Vivo |
In vivo, 2-amino-5-phenylpyridine is not used as a therapeutic agent. Its presence in the diet is a concern for its potential carcinogenic effects. It is metabolically activated to form DNA adducts, which can lead to mutations and cancer.
|
| Enzyme Assay |
Non-cellular assays for 2-amino-5-phenylpyridine include the Ames test, which uses Salmonella typhimurium strains to detect its mutagenic potential. It can also be used in assays to study its metabolic activation by cytochrome P450 enzymes, where it is incubated with liver microsomes and DNA to detect adduct formation.
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| Cell Assay |
2-Amino-5-phenylpyridine is not typically used in standard pharmacological cellular assays. Its effects on cells are studied in the context of genotoxicity. Cellular assays may measure its ability to induce DNA damage (e.g., comet assay, γ-H2AX foci) or mutations in mammalian cell lines.
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| Animal Protocol |
In vivo animal experiments with 2-amino-5-phenylpyridine are conducted to study its carcinogenic potential. Rodents are fed the compound, and the incidence of tumors in various organs is monitored over time. These studies help assess the risk of dietary exposure to heterocyclic amines.
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| ADME/Pharmacokinetics |
2-Amino-5-phenylpyridine is a small, lipophilic molecule with a molecular weight of 170.21. It is soluble in organic solvents. Its pharmacokinetic properties are related to its metabolism and clearance. The compound is typically stored at room temperature.
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| Toxicity/Toxicokinetics |
2-Amino-5-phenylpyridine is considered a potential carcinogen. It is a mutagenic heterocyclic aromatic amine. It is not approved for human therapeutic use. Standard laboratory safety precautions should be followed when handling this compound.
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| References |
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| Additional Infomation |
5-Phenylacetyl-2-pyridylamine is a phenylpyridine.
2-Amino-5-phenylpyridine is a mutagenic heterocyclic aromatic amine formed during the cooking of protein-rich foods. It is considered potentially carcinogenic and is used as a synthetic intermediate. It is also used as a model compound in genotoxicity and food safety research. |
| Molecular Formula |
C₁₁H₁₀N₂
|
|---|---|
| Molecular Weight |
170.21
|
| Exact Mass |
170.084
|
| CAS # |
33421-40-8
|
| PubChem CID |
105097
|
| Appearance |
White to off-white solid powder
|
| Density |
1.133g/cm3
|
| Boiling Point |
323.6ºC at 760mmHg
|
| Melting Point |
132 °C
|
| Flash Point |
175.6ºC
|
| Index of Refraction |
1.625
|
| LogP |
2.912
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
13
|
| Complexity |
152
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
OAPVIBHQRYFYSE-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C11H10N2/c12-11-7-6-10(8-13-11)9-4-2-1-3-5-9/h1-8H,(H2,12,13)
|
| Chemical Name |
5-phenylpyridin-2-amine
|
| Synonyms |
2Amino5phenylpyridine; 2 Amino 5 phenylpyridine
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~587.51 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (14.69 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.69 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.69 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.8751 mL | 29.3755 mL | 58.7510 mL | |
| 5 mM | 1.1750 mL | 5.8751 mL | 11.7502 mL | |
| 10 mM | 0.5875 mL | 2.9375 mL | 5.8751 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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