| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
2,4-Diacetylphloroglucinol targets microbial pathogens through its antimicrobial activity. The compound exerts its antimicrobial effects by disrupting cellular membranes and inhibiting the growth of pathogens. It is a polyketide antibiotic produced by Pseudomonas fluorescens strain CHAO, where it functions as a pathogen suppressor. The compound displays broad-spectrum toxicity against bacteria, fungi, and plants. It is used for biocontrol of soilborne diseases in agriculture.
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| ln Vitro |
2,4-Diacetylphloroglucinol exhibits broad-spectrum antimicrobial activity against bacteria, fungi, and plants. It is a potent antibiotic and antifungal agent produced by Pseudomonas species. The compound displays antiviral activity. It acts as a pathogen suppressor and shows inhibitory effects on microbial growth. The compound's antimicrobial mechanism involves disrupting cellular membranes and inhibiting pathogen growth. It is used for biocontrol of soilborne diseases in agriculture.
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| ln Vivo |
In vivo, 2,4-diacetylphloroglucinol is produced by Pseudomonas fluorescens in the rhizosphere, where it provides biocontrol against plant pathogens. The compound has been studied for its effectiveness in protecting crops from soilborne diseases. It exhibits broad-spectrum antimicrobial activity in vivo, inhibiting the growth of various plant pathogens. The compound is not used as a therapeutic agent for humans but is studied for its agricultural applications.
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| Enzyme Assay |
In vitro antimicrobial assays for 2,4-diacetylphloroglucinol typically involve testing the compound against various bacterial and fungal pathogens using disc diffusion or broth microdilution methods. The compound is dissolved in appropriate solvents (ethanol or DMSO) and serially diluted in growth media. The minimum inhibitory concentration (MIC) is determined by measuring the lowest concentration that inhibits visible growth. Antiviral activity is assessed using cell-based assays with appropriate virus models. The compound's activity is compared to standard antibiotics.
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| Cell Assay |
In vitro cellular assays for 2,4-diacetylphloroglucinol are primarily conducted in the context of its antimicrobial activity. Bacterial or fungal cells are treated with the compound at concentrations ranging from 0.1 to 100 μg/mL. Cell viability is assessed by colony counting or optical density measurements. The compound's effects on cell membrane integrity can be assessed using fluorescent dyes or by measuring leakage of cellular contents. The compound is dissolved in DMSO or ethanol and diluted in culture media.
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| Animal Protocol |
In vivo animal studies for 2,4-diacetylphloroglucinol are not typically performed, as the compound is used for agricultural biocontrol rather than as a therapeutic agent for humans. Studies are typically conducted in plant systems to evaluate the compound's effectiveness in protecting crops from pathogens. The compound is applied to soil or plants, and disease incidence is monitored. The compound is not intended for human therapeutic use.
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| ADME/Pharmacokinetics |
2,4-Diacetylphloroglucinol has a molecular weight of 210.19 g/mol and formula C10H10O5. The compound is a solid at room temperature and should be stored frozen at -20°C. It is soluble in organic solvents such as DMSO and ethanol. Detailed PK parameters are not applicable for this compound, as it is used for agricultural applications rather than as a therapeutic agent.
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| Toxicity/Toxicokinetics |
Toxicity data for 2,4-diacetylphloroglucinol indicate that it has broad-spectrum toxicity against bacteria, fungi, and plants. The compound is intended for research and agricultural use only and is not approved for human therapeutic applications. Appropriate safety precautions should be taken when handling. The compound's antimicrobial activity suggests potential toxicity to microbial systems, which is the basis for its biocontrol applications.
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| References | |
| Additional Infomation |
2,4-Dacetyl-Resorcinol is a phloroglucinol derivative in which two hydrogen atoms on the resorcinol ring are replaced by acetyl groups. It is both a bacterial metabolite and an antifungal agent. It is a diketone, aromatic ketone, methyl ketone, and phloroglucinol. Functionally, it is related to resorcinol. It is the conjugate acid of 2,4-Dacetyl-Resorcinol (1-). 2,4-Dacetyl-Resorcinol has been reported in Pseudomonas, Pseudomonas fluorescens, and Pseudomonas putida, with relevant data available.
2,4-Diacetylphloroglucinol (CAS 2161-86-6) is a natural phenol and polyketide antibiotic produced by Pseudomonas fluorescens. It has a molecular weight of 210.19 g/mol and formula C10H10O5. The compound exhibits broad-spectrum antimicrobial activity and is used for biocontrol of plant pathogens. It displays antiviral activity and inhibitory effects on bacteria, fungi, and plants. The compound is also known as DAPG. It is not approved for human therapeutic use. |
| Molecular Formula |
C10H10O5
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|---|---|
| Molecular Weight |
210.1834
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| Exact Mass |
210.053
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| CAS # |
2161-86-6
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| PubChem CID |
16547
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.422g/cm3
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| Boiling Point |
374.7ºC at 760mmHg
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| Melting Point |
164ºC
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| Flash Point |
194.6ºC
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| Vapour Pressure |
3.81E-06mmHg at 25°C
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| Index of Refraction |
1.62
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| LogP |
1.208
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
15
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| Complexity |
249
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
PIFFQYJYNWXNGE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H10O5/c1-4(11)8-6(13)3-7(14)9(5(2)12)10(8)15/h3,13-15H,1-2H3
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| Chemical Name |
1-(3-acetyl-2,4,6-trihydroxyphenyl)ethanone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~475.78 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.89 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.89 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.89 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7578 mL | 23.7891 mL | 47.5783 mL | |
| 5 mM | 0.9516 mL | 4.7578 mL | 9.5157 mL | |
| 10 mM | 0.4758 mL | 2.3789 mL | 4.7578 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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