| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| ln Vitro |
In rat hepatic stellate cells (HSCs-T6), 11α-Methoxysaikosaponin F was evaluated for its effect on cell viability. The compound showed an IC50 value of 387.70 ± 31.75 μM for HSCs-T6 viability after 24 hours of treatment. No inhibitory activity (or not determined) was observed against TGF-β1-stimulated HSCs-T6 proliferation under the tested conditions (up to 100 μM). [1]
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| Cell Assay |
The effect of 11α-Methoxysaikosaponin F on the viability of HSCs-T6 cells was assessed using the MTS assay. Cells were seeded into 96-well plates at a density of 8×10³ cells per well and incubated at 37°C in a 5% CO₂ incubator for 24 hours. After washing twice with PBS, the cells were incubated with different concentrations of the compound (200, 300, 400, 500, and 600 μM) for 24 hours at 37°C in a 5% CO₂ incubator. Then, 20 μL of MTS reagent was added to each well, and the cells were incubated for another 3 hours. Absorbance was measured at 492 nm using a microplate reader. The IC50 value was calculated. [1]
The inhibitory effect of 11α-Methoxysaikosaponin F on TGF-β1-stimulated HSCs-T6 was also evaluated. Cells were seeded at 8×10³ cells per well in 96-well plates and incubated for 24 hours, then washed with PBS and serum-starved overnight. Subsequently, cells were incubated with the compound at concentrations of 1, 25, 50, 75, and 100 μM, accompanied by stimulation with 10 ng/mL TGF-β1 for 24 hours at 37°C in a 5% CO₂ incubator. After adding 20 μL MTS reagent and incubating for another 3 hours, absorbance was measured at 492 nm. No inhibitory IC50 value was reported for this compound in the stimulated assay. [1] |
| References | |
| Additional Infomation |
11α-Methoxysaikosaponin F (compound 22) is a triterpenoid saponin isolated from Bupleurum marginatum Wall.ex DC (ZYCH). It was one of 25 compounds separated from the plant, among which 21 compounds were isolated for the first time from this species. The compound was obtained from the n-BuOH fraction via silica gel column chromatography and preparative HPLC (ACN-H2O, 28:72). [1]
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| Molecular Formula |
C49H82O18
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|---|---|
| Molecular Weight |
959.1646
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| Exact Mass |
958.55
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| CAS # |
104109-37-7
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| PubChem CID |
21637637
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| Appearance |
White to off-white solid powder
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| Density |
1.38±0.1 g/cm3(Predicted)
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| Boiling Point |
1007.2±65.0 °C(Predicted)
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| Source |
Originated from plants: Compositae Carphephorus corymbosus (Nutt.) Torr. & A.Gray
Originated from plants: Umbelliferae |
| LogP |
1.5
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| Hydrogen Bond Donor Count |
11
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| Hydrogen Bond Acceptor Count |
18
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
67
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| Complexity |
1780
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| Defined Atom Stereocenter Count |
25
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| SMILES |
C[C@H]1[C@@H]([C@H]([C@H]([C@@H](O1)O[C@@H]2[C@H](O[C@H]([C@@H]([C@H]2O)O)O[C@H]3CC[C@]4([C@H](C3(C)C)CC[C@@]5([C@@H]4[C@@H](C=C6[C@]5(C[C@@H]([C@@]7([C@H]6CC(CC7)(C)C)CO)O)C)OC)C)C)CO[C@H]8[C@@H]([C@H]([C@@H]([C@H](O8)CO)O)O)O)O)O)O
|
| InChi Key |
QUBQEHLOFWDOPC-OZRHACRWSA-N
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| InChi Code |
InChI=1S/C49H82O18/c1-22-31(53)33(55)37(59)42(63-22)67-39-27(20-62-41-36(58)34(56)32(54)26(19-50)64-41)65-43(38(60)35(39)57)66-30-11-12-46(6)28(45(30,4)5)10-13-47(7)40(46)25(61-9)16-23-24-17-44(2,3)14-15-49(24,21-51)29(52)18-48(23,47)8/h16,22,24-43,50-60H,10-15,17-21H2,1-9H3/t22-,24-,25+,26+,27+,28-,29-,30-,31-,32+,33+,34-,35+,36+,37+,38+,39+,40+,41+,42-,43-,46-,47+,48+,49+/m0/s1
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| Chemical Name |
(2S,3R,4R,5R,6S)-2-[(2R,3S,4R,5R,6R)-6-[[(3S,4aR,6aR,6bS,8S,8aS,12aS,14R,14aR,14bS)-8-hydroxy-8a-(hydroxymethyl)-14-methoxy-4,4,6a,6b,11,11,14b-heptamethyl-1,2,3,4a,5,6,7,8,9,10,12,12a,14,14a-tetradecahydropicen-3-yl]oxy]-4,5-dihydroxy-2-[[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-3-yl]oxy-6-methyloxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~52.1 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (1.30 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (1.30 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.25 mg/mL (1.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0426 mL | 5.2129 mL | 10.4258 mL | |
| 5 mM | 0.2085 mL | 1.0426 mL | 2.0852 mL | |
| 10 mM | 0.1043 mL | 0.5213 mL | 1.0426 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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