| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
10074-A4 has 15.1 μM IC50 inhibitory action against HL-60 cells[1]. In HL-60 cells, 10074-A4 (25-50 μM; 24 hours) dose-dependently stops the cell cycle in the S phase. The c-Myc target genes CDK4 and CCND2 had their mRNA levels inhibited by 10074-A4 [1]. It is possible to characterize 10074-A4's binding behavior to c-Myc370-409 as a "ligand cloud" because it can bind to the peptide chain at several locations. The c-Myc370-409 peptide's structure is still dynamic in its bound state. Different locations throughout the c-Myc370-409 chain bind to the 10074-A4 ligand with varying intensities [2].
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| Cell Assay |
Cell viability assay [1]
Cell Types: HL-60 Cell Tested Concentrations: 25 μM, 50 μM Incubation Duration: 24 hrs (hours) Experimental Results: The cell cycle was arrested in S phase. |
| References |
| Molecular Formula |
C18H14CL2N2O3S
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|---|---|
| Molecular Weight |
409.28
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| Exact Mass |
408.01
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| CAS # |
312631-87-1
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| PubChem CID |
2836204
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| Appearance |
White to off-white solid powder
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| LogP |
4.095
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
26
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| Complexity |
555
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
SCNHANGMZXFWOH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H14Cl2N2O3S/c19-10-1-3-15-13(5-10)14-6-11(20)2-4-16(14)21(15)7-12(23)8-22-17(24)9-26-18(22)25/h1-6,12,23H,7-9H2
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| Chemical Name |
3-[3-(3,6-dichlorocarbazol-9-yl)-2-hydroxypropyl]-1,3-thiazolidine-2,4-dione
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| Synonyms |
10074-A4 10074A4 10074 A4
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~305.41 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.08 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.08 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4433 mL | 12.2166 mL | 24.4332 mL | |
| 5 mM | 0.4887 mL | 2.4433 mL | 4.8866 mL | |
| 10 mM | 0.2443 mL | 1.2217 mL | 2.4433 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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