| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
1-Naphthyl acetate does not have a traditional therapeutic target. It is a substrate for esterases and other hydrolytic enzymes. Its mechanism of action is based on enzymatic hydrolysis: esterases cleave the acetate ester bond, releasing 1-naphthol. The released 1-naphthol can then react with diazonium salts to form a colored precipitate, enabling the detection and localization of enzyme activity in histological samples. It is used as a chromogenic substrate in various biochemical assays to measure esterase activity.
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|---|---|
| ln Vitro |
In vitro, 1-Naphthyl acetate is used as a chromogenic substrate for detecting esterase activity in biological samples. It is employed in rapid staining methods for the identification of macrophages. The enzymatic hydrolysis of 1-naphthyl acetate by esterases releases 1-naphthol, which can be detected colorimetrically. It is not typically used as a therapeutic agent with pharmacological activity, but rather as a biochemical reagent for research and diagnostic applications.
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| ln Vivo |
1-Naphthyl acetate is not used as a therapeutic agent in vivo. It is a biochemical reagent used in research and diagnostic applications. No in vivo pharmacological activity data is relevant. Its primary applications are in histology and biochemistry for the detection of enzyme activity.
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| Enzyme Assay |
For in vitro cell-free assays, 1-Naphthyl acetate is used as a substrate for measuring esterase activity. The assay involves incubating the compound with the enzyme sample (e.g., esterase, lipase) in a suitable buffer. The enzymatic hydrolysis of 1-naphthyl acetate releases 1-naphthol, which can be detected by adding a diazonium salt (such as Fast Blue RR or Fast Red TR) to form a colored precipitate. The intensity of the color, measured spectrophotometrically, is proportional to the enzyme activity.
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| Cell Assay |
For in vitro cellular assays, 1-Naphthyl acetate is used in histochemical staining procedures for the identification of macrophages and other cell types. Cells or tissue sections are incubated with a solution containing 1-naphthyl acetate and a diazonium salt. Esterase enzymes present in the cells hydrolyze the substrate, releasing 1-naphthol, which couples with the diazonium salt to form an insoluble colored precipitate at the site of enzyme activity. Stained cells are then visualized by light microscopy.
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| Animal Protocol |
1-Naphthyl acetate is not used in animal models as a therapeutic agent. It is a biochemical reagent used in research and diagnostic applications. In vivo administration is not typically performed for this compound. Its use is limited to in vitro applications such as histochemical staining and enzyme assays.
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| ADME/Pharmacokinetics |
1-Naphthyl acetate (CAS 830-81-9) has a molecular formula of C12H10O2 and a molecular weight of 186.21 g/mol. It is also known as 1-acetoxynaphthalene, alpha-naphthyl acetate, and acetic acid-1-naphthyl ester. Appearance: white solid. Melting point: 41-43°C. Boiling point: 310°C. Vapor pressure: 0.0006 (25°C). Solubility: insoluble in water. Storage: room temperature, in a cool and dark place (<15°C). Purity: typically ≥98%.
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| Toxicity/Toxicokinetics |
No toxicity data is publicly available. As a laboratory reagent, 1-Naphthyl acetate should be handled with standard laboratory safety precautions. It is not intended for human consumption or therapeutic use.
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| References | |
| Additional Infomation |
1-Naphthyl acetate is a research-grade biochemical reagent and is not approved for therapeutic use. It is primarily used as a chromogenic substrate for detecting esterase activity in histological and biochemical applications. It is used in rapid staining methods for the identification of macrophages. Its mechanism involves enzymatic hydrolysis by esterases, releasing 1-naphthol which can be detected colorimetrically. No clinical trials have been reported.
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| Molecular Formula |
C12H10O2
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|---|---|
| Molecular Weight |
186.2066
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| Exact Mass |
186.068
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| CAS # |
830-81-9
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| PubChem CID |
13247
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
310.0±11.0 °C at 760 mmHg
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| Melting Point |
43-46 °C(lit.)
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| Flash Point |
110.4±8.2 °C
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| Vapour Pressure |
0.0±0.7 mmHg at 25°C
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| Index of Refraction |
1.604
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| LogP |
2.79
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
14
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| Complexity |
212
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VGKONPUVOVVNSU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H10O2/c1-9(13)14-12-8-4-6-10-5-2-3-7-11(10)12/h2-8H,1H3
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| Chemical Name |
naphthalen-1-yl acetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~537.03 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.43 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (13.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.3703 mL | 26.8514 mL | 53.7028 mL | |
| 5 mM | 1.0741 mL | 5.3703 mL | 10.7406 mL | |
| 10 mM | 0.5370 mL | 2.6851 mL | 5.3703 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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