| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| 500mg |
| Animal Protocol |
- Male rats were utilized for the study. Rats were fed diets supplemented with meats from different sources (beef, pork, chicken) or exogenous 3-methylhistidine. Urine samples were continuously collected for 24-72 hours post-feeding, acidified immediately, and stored at -20°C for subsequent analysis. 1-Methyl-L-histidine in urine was quantified using chromatographic techniques to verify its excretion as a marker of exogenous intake [1]
|
|---|---|
| ADME/Pharmacokinetics |
1-Methyl-L-histidine is a urinary excretory metabolite that can be used as a qualitative indicator of exogenous 3-methylhistidine intake. After oral intake of exogenous 3-methylhistidine or meat from various sources (beef, pork, chicken), 1-methyl-L-histidine is excreted in urine without significant metabolic conversion. 1-Methyl-L-histidine can be detected in urine within 24 hours after intake, and its presence can specifically confirm the intake of protein containing exogenous 3-methylhistidine [1].
|
| References | |
| Additional Infomation |
N(tele)-methyl-L-histidine is an L-histidine derivative with a methyl group at the N(tele) position. It is a human metabolite. It is an L-histidine derivative and a non-protein L-α-amino acid. It is a zwitterionic tautomer of N(tele)-methyl-L-histidine. 1-Methylhistidine is present in or produced by Escherichia coli (K12 strain, MG1655 strain). 1-Methyl-L-histidine has also been reported in Prunus species, with relevant data. 1-Methylhistidine is a histidine derivative with a methyl group attached to the nitrogen atom at position 1; it is a product of the metabolism of the dipeptide gooserine. The level of 1-methylhistidine in urine may indicate myofibrillar protein degradation or a high-protein diet.
- 1-methyl-L-histidine is a naturally occurring methylated histidine derivative[1] - Since mammals cannot synthesize 1-methylhistidine endogenously, it is a specific qualitative biomarker for exogenous 3-methylhistidine intake and meat (beef, pork, chicken) consumption. Its urinary excretion directly reflects the intake of protein rich in 3-methylhistidine[1]. |
| Molecular Formula |
C7H11N3O2
|
|---|---|
| Molecular Weight |
169.18114
|
| Exact Mass |
169.085
|
| CAS # |
332-80-9
|
| PubChem CID |
92105
|
| Appearance |
White to off-white solid powder
|
| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
415.0±35.0 °C at 760 mmHg
|
| Melting Point |
~240 °C (dec.)(lit.)
|
| Flash Point |
204.8±25.9 °C
|
| Vapour Pressure |
0.0±1.0 mmHg at 25°C
|
| Index of Refraction |
1.613
|
| LogP |
-1.31
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
12
|
| Complexity |
174
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
CN1C=C(N=C1)C[C@@H](C(=O)O)N
|
| InChi Key |
BRMWTNUJHUMWMS-LURJTMIESA-N
|
| InChi Code |
InChI=1S/C7H11N3O2/c1-10-3-5(9-4-10)2-6(8)7(11)12/h3-4,6H,2,8H2,1H3,(H,11,12)/t6-/m0/s1
|
| Chemical Name |
(2S)-2-amino-3-(1-methylimidazol-4-yl)propanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~125 mg/mL (~738.86 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (591.09 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9109 mL | 29.5543 mL | 59.1086 mL | |
| 5 mM | 1.1822 mL | 5.9109 mL | 11.8217 mL | |
| 10 mM | 0.5911 mL | 2.9554 mL | 5.9109 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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