| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| 1g | |||
| Other Sizes |
| Targets |
- 1-Kestose targets intestinal commensal bacteria, specifically Faecalibacterium prausnitzii and Bifidobacteria (acts as a nutrient substrate for these bacteria) [1][2]
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|---|---|
| ln Vitro |
Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium discovered during Crohn's disease gut microbiota study. Bifidobacteria are useful for atopic dermatitis (AD) [1].
- Human fecal microbiota fermentation assay: Fecal samples from healthy adults were diluted and incubated with 1-Kestose (final concentration: 1% w/v) in an anaerobic system at 37°C for 24 hours. Quantitative real-time PCR (qPCR) showed Faecalibacterium prausnitzii increased by ~2.5 log10 copies/g feces and Bifidobacteria by ~1.8 log10 copies/g feces vs. control. High-performance liquid chromatography (HPLC) revealed elevated short-chain fatty acids (SCFAs): acetate (+35 mM), propionate (+12 mM), butyrate (+8 mM) [1][2] |
| ln Vivo |
- Human intervention trial: 30 healthy adults (15 males, 15 females, 20–45 years) received 1-Kestose (10 g/day, dissolved in 200 mL water, oral, once daily) for 2 weeks (2-week washout). Fecal qPCR showed Faecalibacterium prausnitzii (+2.1 log10 copies/g feces) and Bifidobacteria (+1.5 log10 copies/g feces) increased vs. baseline, returning to baseline after washout. HPLC showed increased SCFAs: acetate (+28 mM), propionate (+9 mM), butyrate (+6 mM) [1][2]
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| ADME/Pharmacokinetics |
Absorption: 1-Fructooligosaccharide is not digested by upper digestive enzymes, nor is it absorbed in the small intestine, and reaches the large intestine intact[1][2]
- Metabolism: In the large intestine, it is fermented by intestinal flora (including Clostridium plasminogen salina and Bifidobacterium) into short-chain fatty acids (acetic acid, propionic acid, butyric acid), carbon dioxide and hydrogen (not involving human metabolic enzymes)[1][2] - Excretion: Absorbed short-chain fatty acids are used by colon cells; unabsorbed short-chain fatty acids are excreted through feces, and gaseous products (carbon dioxide, hydrogen) are excreted through intestinal gas[1][2] |
| Toxicity/Toxicokinetics |
- Human toxicity: No adverse reactions (bloating, diarrhea, abdominal pain) were reported in subjects who took 1-sucrose trisaccharide (10 g/day for 2 weeks); blood routine, liver function (ALT, AST) and kidney function (creatinine, blood urea nitrogen) were normal [1][2]
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| References |
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| Additional Infomation |
1-Fructotriose is a trisaccharide found in vegetables, consisting of β-D-fructofuranose with β-D-fructofuranose and α-D-glucopyranose residues at positions 1 and 2, respectively.
1-Fructotriose has been reported to be found in burdock (Arctium umbrosum), Daphnia pulex, and other organisms with relevant data. - 1-Fructotriose is the smallest component of fructooligosaccharides (FOS) with the structure glucose-fructose-fructose (GFF)[1][2] - It is a prebiotic (an indigestible food component that selectively stimulates beneficial gut bacteria) and has a stronger growth-stimulating effect on Faecalibacterium prausnitzii than other fructooligosaccharides (e.g., nitrates)[1][2] - Its probiotic effect stems from its role as a specific nutrient for Faecalibacterium prausnitzii. Promotes the growth of Clostridium praosporum and Bifidobacterium and the production of short-chain fatty acids (SCFAs) (SCFAs maintain intestinal barrier function and regulate intestinal immunity) [1][2] |
| Molecular Formula |
C18H32O16
|
|---|---|
| Molecular Weight |
504.4371
|
| Exact Mass |
504.169
|
| CAS # |
470-69-9
|
| PubChem CID |
440080
|
| Appearance |
White to off-white solid powder
|
| Density |
1.82g/cm3
|
| Boiling Point |
902.9ºC at 760mmHg
|
| Flash Point |
499.9ºC
|
| Vapour Pressure |
0mmHg at 25°C
|
| Index of Refraction |
1.679
|
| LogP |
-5.5
|
| Hydrogen Bond Donor Count |
11
|
| Hydrogen Bond Acceptor Count |
16
|
| Rotatable Bond Count |
9
|
| Heavy Atom Count |
34
|
| Complexity |
670
|
| Defined Atom Stereocenter Count |
13
|
| SMILES |
C([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@]2([C@H]([C@@H]([C@H](O2)CO)O)O)CO[C@]3([C@H]([C@@H]([C@H](O3)CO)O)O)CO)O)O)O)O
|
| InChi Key |
VAWYEUIPHLMNNF-OESPXIITSA-N
|
| InChi Code |
InChI=1S/C18H32O16/c19-1-6-9(23)12(26)13(27)16(31-6)34-18(15(29)11(25)8(3-21)33-18)5-30-17(4-22)14(28)10(24)7(2-20)32-17/h6-16,19-29H,1-5H2/t6-,7-,8-,9-,10-,11-,12+,13-,14+,15+,16-,17-,18+/m1/s1
|
| Chemical Name |
(2R,3R,4S,5S,6R)-2-[(2S,3S,4S,5R)-2-[[(2R,3S,4S,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~198.24 mM)
H2O : ~83.33 mg/mL (~165.19 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.96 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.96 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (4.96 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (198.24 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9824 mL | 9.9120 mL | 19.8240 mL | |
| 5 mM | 0.3965 mL | 1.9824 mL | 3.9648 mL | |
| 10 mM | 0.1982 mL | 0.9912 mL | 1.9824 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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