| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| 250mg |
|
||
| 500mg | |||
| Other Sizes |
| Targets |
γ-GT targets gamma-glutamyltransferase (GGT), a membrane-bound enzyme that catalyzes the transfer of γ-glutamyl groups from glutathione to amino acids or peptides. As a substrate, it is cleaved by GGT to release a chromogenic or fluorogenic product for enzyme activity measurement.
|
|---|---|
| ln Vitro |
γ-GT serves as a chromogenic substrate for gamma-glutamyltransferase (GGT), enabling the quantitative measurement of GGT enzyme activity in biochemical assays. Upon enzymatic cleavage, it produces a measurable signal (colorimetric or fluorometric) that correlates with GGT activity in the sample.
|
| ln Vivo |
As a biochemical substrate, γ-GT is not evaluated for in vivo activity in animal models. Its application is in diagnostic enzyme activity assays rather than therapeutic in vivo studies.
|
| Enzyme Assay |
In vitro enzyme assays using γ-GT involve incubating the substrate with GGT enzyme or biological samples containing GGT (e.g., serum, tissue homogenates) in a suitable buffer. The enzymatic reaction releases a chromogenic product (e.g., p-nitroaniline) that is measured spectrophotometrically at 405 nm to quantify GGT activity.
|
| Cell Assay |
In vitro cell-based assays using γ-GT are performed to measure cellular GGT activity. Cells are lysed and the lysate is incubated with γ-GT substrate, followed by spectrophotometric measurement of the released chromophore. Alternatively, live cells can be incubated with the substrate and fluorescence or absorbance is measured to assess cell surface GGT activity.
|
| Animal Protocol |
In vivo animal studies are not typically performed with γ-GT as it is a diagnostic substrate rather than a therapeutic agent. It may be used ex vivo to measure GGT activity in tissue samples collected from animal studies to assess glutathione metabolism, oxidative stress, or liver function.
|
| ADME/Pharmacokinetics |
γ-GT has a molecular weight of 328.28 g/mol and formula C₁₂H₁₆N₄O₇. It is a synthetic peptide-derived substrate that is soluble in aqueous buffers. As a diagnostic reagent, it does not exhibit systemic pharmacokinetics. It should be stored at -20°C.
|
| Toxicity/Toxicokinetics |
γ-GT is considered low in toxicity as a biochemical reagent. Standard laboratory safety precautions should be followed. No significant toxicity has been reported as it is used in small quantities in diagnostic assays.
|
| Additional Infomation |
γ-GT is a widely used diagnostic substrate for measuring gamma-glutamyltransferase (GGT) activity in serum and other biological samples, with applications in liver function testing and assessment of glutathione metabolism, oxidative stress, and related metabolic or disease processes. Not approved for therapeutic use.
|
| Molecular Formula |
C12H12N3O7-.H4N+
|
|---|---|
| Molecular Weight |
328.27804
|
| Exact Mass |
328.101
|
| CAS # |
63699-78-5
|
| PubChem CID |
104545
|
| Appearance |
Light yellow to yellow solid powder
|
| Boiling Point |
685.7ºC at 760 mmHg
|
| Melting Point |
187ºC ± 2.0ºC
|
| Flash Point |
368.5ºC
|
| LogP |
2.044
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
22
|
| Complexity |
462
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
C1=CC(=C(C=C1NC(=O)CC[C@@H](C(=O)O)N)C(=O)O)[N+](=O)[O-]
|
| InChi Key |
AHFFWTMLFDZSOF-QMMMGPOBSA-N
|
| InChi Code |
InChI=1S/C12H13N3O7/c13-8(12(19)20)2-4-10(16)14-6-1-3-9(15(21)22)7(5-6)11(17)18/h1,3,5,8H,2,4,13H2,(H,14,16)(H,17,18)(H,19,20)/t8-/m0/s1
|
| Chemical Name |
5-[[(4S)-4-amino-4-carboxybutanoyl]amino]-2-nitrobenzoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ≥ 30 mg/mL (~91.39 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 7.14 mg/mL (21.75 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0462 mL | 15.2309 mL | 30.4618 mL | |
| 5 mM | 0.6092 mL | 3.0462 mL | 6.0924 mL | |
| 10 mM | 0.3046 mL | 1.5231 mL | 3.0462 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
