| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
β-Amyloid (31-35) is the functional cytotoxic domain of the Aβ peptide. β-Amyloid(31-35) promotes the phosphorylation of biotinylated Aβ(1-40), enhances CDK-1 activity, and inhibits the binding of Aβ to cyclin B1. β-Amyloid(31-35) is cytotoxic, and olomoucine suppresses this action in neurons of the differentiated human teratocarcinoma cell line Ntera 2/cl-D1 (NT-2) [1]. Recommendations for Beta-Amyloid Aggregation (This is our suggested approach; it should be adjusted to suit your particular need as it simply offers guidelines). 1. Solid Aβ peptide should be dissolved in cold hexafluoro-2-propanol (HFIP). To achieve monomerization and randomization of the structure, incubate the peptides for a minimum of one hour at room temperature. 2. The HFIP is removed by evaporation and the resultant peptide is kept as a thin film at -20 or -80 °C. 3. The resultant membrane should be dissolved in 5 mM of anhydrous DMSO, vortexed, and diluted with buffer (serum- and phenol-red-free media) to the proper concentration. 4. After that, let the solution age for 48 hours at 4–8°C. The materials were then centrifuged at 14,000g for 10 minutes at 4-8°C; soluble oligomers were in the supernatant. For the studies, the supernatant was diluted 10-200 times. Different approaches are used based on the final application.
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| References |
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| Molecular Formula |
C25H47N5O6S
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|---|---|
| Molecular Weight |
545.73558
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| Exact Mass |
545.325
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| CAS # |
149385-65-9
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| PubChem CID |
10816380
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| Appearance |
White to off-white solid powder
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| Density |
1.137 g/cm3
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| Boiling Point |
866.382ºC at 760 mmHg
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| LogP |
3.124
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
18
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| Heavy Atom Count |
37
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| Complexity |
766
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| Defined Atom Stereocenter Count |
6
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| SMILES |
CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)O)N
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| InChi Key |
ZMDGLWRNBGRYQB-ZKHIMWLXSA-N
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| InChi Code |
InChI=1S/C25H47N5O6S/c1-8-15(5)20(26)23(33)30-21(16(6)9-2)24(34)27-13-19(31)28-18(12-14(3)4)22(32)29-17(25(35)36)10-11-37-7/h14-18,20-21H,8-13,26H2,1-7H3,(H,27,34)(H,28,31)(H,29,32)(H,30,33)(H,35,36)/t15-,16-,17-,18-,20-,21-/m0/s1
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| Chemical Name |
(2S)-2-[[(2S)-2-[[2-[[(2S,3S)-2-[[(2S,3S)-2-amino-3-methylpentanoyl]amino]-3-methylpentanoyl]amino]acetyl]amino]-4-methylpentanoyl]amino]-4-methylsulfanylbutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product is not stable in solution, please use freshly prepared working solution for optimal results. (2). Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~5 mg/mL (~9.16 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.81 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (3.81 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8324 mL | 9.1619 mL | 18.3237 mL | |
| 5 mM | 0.3665 mL | 1.8324 mL | 3.6647 mL | |
| 10 mM | 0.1832 mL | 0.9162 mL | 1.8324 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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