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100mg | ||
250mg | ||
500mg | ||
Other Sizes |
ln Vitro |
In chicken embryo fibroblasts (CEFs), neruriminidase-IN-16 (Compound 43b; 48 h) demonstrates anti-influenza viral activity against H5N1 and H5N8, with EC50s of 2.10±0.44 μM and 2.28±0.67 μM, respectively [1]. With EC50s of 0.20±0.01 μM and 11.3±2.5 μM, respectively, neuraminidase-IN-16(48 h) demonstrates anti-influenza viral efficacy against H1N1 and H3N2 in MDCK cells [1].
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ln Vivo |
In some pathogen-free (SPF) embryonated chicken eggs, neruminidase-IN-16 (Compound 43b; 0.039-10 mM; 100 uL) demonstrates anti-influenza viral action [1]. The blood-brain barrier is insurmountable for neurominidase-IN-16 [1].
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Animal Protocol |
Animal/Disease Models: SPF chicken embryonated egg infected with H5N1 and H5N8 virus strains[1]
Doses: 0.039, 0.156, 0.625, 2.5 and 10 mM; 100 uL Route of Administration: A 1 mm diameter hole was drilled into the egg shell on the side where the The embryo was located, the mixture was injected into the egg, and the holes were sealed by wax. Experimental Results: demonstrated 100% protective effect at the highest tested concentration (10 mM). |
References |
[1]. Jia R, et al. Discovery of N-substituted oseltamivir derivatives as novel neuraminidase inhibitors with improved drug resistance profiles and favorable drug-like properties. Eur J Med Chem. 2023 Apr 5;252:115275.
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Molecular Formula |
C26H35FN2O4
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Molecular Weight |
458.57
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.1807 mL | 10.9035 mL | 21.8069 mL | |
5 mM | 0.4361 mL | 2.1807 mL | 4.3614 mL | |
10 mM | 0.2181 mL | 1.0903 mL | 2.1807 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.