| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| ln Vitro |
Sturlin (1-50 μM; 72 hours) showed antiproliferative activity against human lung cancer A549 cells, ovarian cancer A2780 cells, breast cancer SK-BR-3 cells, and MCF-7 cells. Low concentrations (1 μM, 5 μM) had little or no effect on cell proliferation or only slightly delayed it, while high concentrations (10 μM, 20 μM, 50 μM) significantly inhibited cell proliferation [2]. Sturlin (2.5-20 μM; 24 hours) could induce apoptosis in Jurkat and MOLT-4 leukemia cells, with both early and late apoptosis rates increasing in a dose-dependent manner, and DNA fragmentation could be detected by TUNEL [2].
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| Cell Assay |
ELISA detection [2]
Cell Types: Jurkat, MOLT-4, Raji, HL-60, U-937, HEL 92.1.7, A549, A2780, SK-BR-3, MCF-7 Tested Concentrations: 1 μM, 5 μM, 10 μM, 15 μM, 20 μM, 50 μM Incubation Duration: 24 hours, 48 hours, 72 hours Experimental Results: The ELISA reduced the viability and proliferation of leukemia cells in a dose-dependent manner within 24 hours, with a more significant reduction effect after 48 hours. For adherent cell lines, low concentrations (1 μM, 5 μM) had no significant effect on cell proliferation or only slightly delayed it, while high concentrations (10 μM, 20 μM, 50 μM) showed a strong anti-proliferative effect within 72 hours. The IC50 values of scoulerine against human leukemia cells, determined by the XTT assay, ranged from 2.7 to 6.5 μM. The IC50 values were: 2.7 ± 0.1 μM (Jurkat cells), 4.2 ± 0.2 μM (HL-60 cells), 4.7 ± 0.2 μM (MOLT-4 cells), 4.8 ± 0.3 μM (U-937 cells), 6.3 ± 0.8 μM (HEL 92.1.7 cells), and 6.5 ± 0.7 μM (Raji cells). Cell viability assays; Western blot analysis; cell proliferation assays; apoptosis assays; cytotoxicity assays; cell cycle analysis; RT-PCR; autophagy assays; immunofluorescence assays; cell differentiation assays; cell invasion assays; cell migration assays; and real-time quantitative PCR. ELISA detection [2] Cell Types: A549 Tested Concentrations: 5 μM, 10 μM, 20 μM Incubation Duration: 24 hours Experimental Results: At concentrations of 10 μM and 20 μM, the microtubule structure was disrupted, leading to dense aggregation of microtubules around the cell nucleus and a decrease in microtubule density around the cell periphery. At a concentration of 5 μM, the microtubule network remained almost intact, similar to the control group cells. |
| References |
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| Molecular Formula |
C19H22CLNO4
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|---|---|
| Molecular Weight |
363.84
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| CAS # |
51486-68-1
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| Related CAS # |
Scoulerine
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| Appearance |
Typically exists as solids at room temperature
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| SMILES |
OC1=C(OC)C=C(CCN2CC3=C(O)C(OC)=CC=C3C[C@]24[H])C4=C1.Cl
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| Synonyms |
(-)-Scoulerine hydrochloride; Discretamine hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7485 mL | 13.7423 mL | 27.4846 mL | |
| 5 mM | 0.5497 mL | 2.7485 mL | 5.4969 mL | |
| 10 mM | 0.2748 mL | 1.3742 mL | 2.7485 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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