| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Fluchlorfon (5-20 μg/mL; 24-72 h) exhibited dose- and time-dependent cytotoxicity in CHO and MEF cells[2]. Fluchlorfon (2.5-20.0 μg/mL; 4-12 h) induced statistically significant, time-dependent chromosomal aberrations in CHO cells[2]. Fluchlorfon (1.0-20.0 μg/mL; 12 h) significantly increased the level of sister chromatid exchange in CHO cells[2]. Fluchlorfon (5-20 μg/mL; 30 h) had a dose-dependent inhibitory effect on DNA synthesis in synchronized MEF cells without affecting thymidine transport[2]. Fluchlorfon (5-20 μg/mL; 6 h) reduced the level of DNA synthesis in synchronized MEF cells by nearly 50% 12 hours after treatment without affecting thymidine transport[2]. Fluchlor (5-20 μg/mL; 24-72 hours) can induce apoptosis in CHO cells, which is manifested by the formation of DNA ladder bands and typical nuclear changes [2].
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|---|---|
| ln Vivo |
Fluchlor (0.5–2 mg/L; water immersion; daily; 96 hours) can produce dose-dependent developmental toxicity in zebrafish embryos, including reduced survival (LC50 = 2.004 mg/L), morphological defects, neurotoxicity, cardiotoxicity, liver damage, and increased brain cell apoptosis [1].
|
| Cell Assay |
Cytotoxicity assay [2]
Cell Types: Chinese hamster ovary (CHO) cells, mouse embryonic fibroblast (MEF) cells Tested Concentrations: 5, 10, 15 and 20 μg/mL Incubation Duration: 24 hours; 48 hours; 72 hours Experimental Results: Cell viability decreased in a dose-dependent manner for both cell lines, and CHO cells were more toxic than MEF cells. After treatment with 20 μg/mL for 72 hours, the viability of CHO cells decreased to 50%. After treatment with 20 μg/mL for 72 hours, the viability of MEF cells decreased to 50%. Apoptosis Analysis [2] Cell Types: Chinese hamster ovary (CHO) cells Tested Concentrations: 5, 10, and 20 μg/mL Incubation Duration: 24 hours; 48 hours; 72 hours Experimental Results: The DNA of the treated CHO cells showed characteristic DNA ladder bands in agarose gel electrophoresis. Microscopic observation showed apoptotic features, including cell shrinkage and nuclear fragmentation. Apoptosis was induced after treatment with a concentration of 20 μg/mL for 72 hours. |
| Animal Protocol |
Animal/Disease Models: Transgenic zebrafish (olig2:dsRed, cmlc2:dsRed, lfabp:dsRed; elastase:GFP); wild-type zebrafish (raised at 26-28.5℃ under a 13-hour light/11-hour dark cycle, 8 hours post-fertilization) [1]
Doses: 0.5 mg/L; 1 mg/L; 2 mg/L Route of Administration: Water immersion; daily; 96 hours Experimental Results: Significantly increased the number of apoptotic cells in the zebrafish brain. It caused developmental toxicity to multiple organs (nervous system, heart, liver, pancreas) of zebrafish embryos. |
| References |
|
| Molecular Formula |
C12H13CLF3N3O4
|
|---|---|
| Molecular Weight |
355.70
|
| CAS # |
33245-39-5
|
| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
ClCCN(CCC)C1=C(C=C(C(F)(F)F)C=C1[N+]([O-])=O)[N+]([O-])=O
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8114 mL | 14.0568 mL | 28.1136 mL | |
| 5 mM | 0.5623 mL | 2.8114 mL | 5.6227 mL | |
| 10 mM | 0.2811 mL | 1.4057 mL | 2.8114 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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