| ln Vitro |
AD-BChE/HClO (AND gate probe, probe S1) (10 μM; maximum 80 minutes) is an AND gate probe that only produces strong fluorescence when BChE and HClO are present simultaneously. In cell-free biochemical analysis, the detection limit of BChE is 8.28 U/L and the detection limit of HClO is 3.09 μM [1]. AD-BChE/HClO (AND gate probe, probe S1) (0-100 μM; cytotoxicity assay 24 hours, imaging assay 40 minutes) is non-cytotoxic to PC12 cells at concentrations up to 100 μM and can specifically detect exogenous and endogenous HClO and upregulated BChE. It can also distinguish between Aβ1-42-induced or glutamate-induced AD model PC12 cells and normal cells by two-photon fluorescence imaging [1].
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| Cell Assay |
Cytotoxicity assay [1]
Cell Types: PC12 cells Tested Concentrations: 0-100 μM (cytotoxicity assay); 10 μM (imaging assay); 50-200 μM HClO; 200-500 μM hydrocortisone; 5-20 μM Aβ1-42; 5-20 mM L-glutamate Incubation Duration: 24 hours (cytotoxicity assay; L-glutamate stimulation); 40 minutes (imaging probe incubation); 30 minutes (HClO stimulation); 48 hours (hydrocortisone stimulation; Aβ1-42 stimulation) Experimental Results: After incubation for 24 hours at concentrations of 0 to 100 μM, the survival rate of PC12 cells remained above 80%. The fluorescence intensity increased with the increase of exogenous HClO concentration. LPS-induced production of endogenous HClO leads to increased fluorescence intensity, while ABH treatment reduces this increase. Hydrocortisone-induced upregulation of BChE leads to increased fluorescence intensity, but the BChE inhibitor iso-OMPA significantly reduces this upregulation more than the AChE inhibitor donepezil. In Aβ1-42-induced and glutamate-induced AD cell models, fluorescence intensity is 2-3 times higher than in normal cells, and ABH or iso-OMPA treatment reduces this fluorescence intensity. |
| References |
| Appearance |
Typically exists as solids at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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