| Targets |
Not applicable. 17:1 Lyso PC is a synthetic phospholipid and a biochemical reagent, not a drug targeting a specific protein. It is used as a liposome membrane component. Its "target" is the aqueous cavity or the lipid bilayer of the vesicle. It acts as a surfactant or lipid excipient. It is particularly used to construct liposomes for drug delivery, helping to overcome problems such as inefficient cellular uptake and rapid loss of payloads in the body.
|
|---|---|
| ln Vitro |
17:1 Lyso PC forms liposomes (vesicles with concentric phospholipid bilayer membranes). As a single-chain lipid, it promotes positive membrane curvature due to its inverted-cone molecular shape. The cis double bond at the delta10 position increases acyl chain flexibility and fluidity relative to saturated LPCs, enhancing its ability to induce curvature. It is used to encapsulate highly polar water-soluble payloads in the internal aqueous space, or lipophilic payloads in the lipid bilayer.
|
| ln Vivo |
No specific in vivo data is available for the pure lipid. Its in vivo "activity" is determined by the drug payload it carries. When formulated into liposomes, the drug exhibits a longer circulation half-life and enhanced permeability and retention (EPR) effect in tumors. The lipid itself is biodegradable and helps deliver antisense oligonucleotides to target cells, overcoming issues like nuclease degradation and poor cellular uptake.
|
| Enzyme Assay |
Not applicable. 17:1 Lyso PC is a liposome-forming material, not a test compound for enzyme binding. It is evaluated via biophysical methods. To measure critical micelle concentration (CMC): Dilutions of the lipid in PBS (0.01-100 ug/mL) are mixed with a lipophilic dye (e.g., Nile Red or diphenylhexatriene, DPH). Fluorescence intensity is measured. The CMC is the concentration at which a sharp increase in fluorescence (indicating micelle formation) occurs.
|
| Cell Assay |
Standard cellular uptake assay for liposomes. Liposomes are prepared containing 17:1 Lyso PC and a fluorescent dye (e.g., 0.2% mol Rhodamine-DOPE). Cancer cells (e.g., HeLa or A549) are seeded in 8-well chamber slides (5×10⁴ cells/well). The fluorescent liposomes (10-100 ug/mL) are added to the cells and incubated for 2-24 hours at 37degC. Cells are washed and fixed. Uptake is visualized by confocal microscopy (red fluorescence). Flow cytometry is used to quantify the percentage of fluorescent cells.
|
| Animal Protocol |
Mouse xenograft model for antisense oligonucleotide (ASO) delivery. Nude mice bearing subcutaneous tumor xenografts are injected intravenously with 17:1 Lyso PC liposomes encapsulating the ASO (5-20 mg/kg of ASO). Control groups receive free ASO or empty liposomes. After 24-48 h, mice are sacrificed, and tumors and major organs are harvested. The concentration of the ASO in the tissue homogenates is measured by qPCR or Northern blot. Tumor growth inhibition is monitored.
|
| ADME/Pharmacokinetics |
Not applicable for the pure lipid; PK is dependent on the formulation. For liposomal drugs, the half-life (t1/2) of the encapsulated drug is much longer (hours to days) compared to free drug (minutes). The Volume of Distribution (Vd) is often close to the plasma volume, as liposomes remain in circulation until cleared by the RES (liver/spleen). 17:1 Lyso PC helps stabilize the bilayer and prevent drug leakage.
|
| Toxicity/Toxicokinetics |
17:1 Lyso PC is a standard laboratory chemical. Safety data sheets indicate it is for research use only and should be handled with appropriate PPE (gloves, lab coat, goggles). It is not classified as acutely toxic, but as a synthetic lipid, it is an eye and skin irritant. Avoid dust or aerosol formation. It is not intended for human or veterinary diagnostic or therapeutic use.
|
| References | |
| Additional Infomation |
17:1 Lyso PC is also known as LPC 17:1/0:0. Its molecular formula is C2₅H₅0NO₇P. It is a polar headgroup lipid used in the generation of positively curved membranes, essential for fusion and endocytosis studies. It is soluble in DMSO (100 mg/mL) and ethanol. For research use only. Not for drug, human, or household use.
|
| CAS # |
1246304-62-0
|
|---|---|
| Appearance |
Typically exists as solids at room temperature
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
