| Size | Price | Stock | Qty |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
As a chemical linker for PROTACs, Cbz-N-amido-PEG2-acid does not have a biological target itself. Its function is to serve as a structural bridge in the design of PROTAC molecules. PROTACs are bifunctional molecules that recruit an E3 ubiquitin ligase to a target protein, leading to its ubiquitination and subsequent degradation by the proteasome. The PEG linker provides flexibility and solubility to the PROTAC molecule.
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| ln Vitro |
One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. The intracellular ubiquitin-proteasome system is utilized by PROTACs to specifically destroy target proteins[1].
The compound has no direct biological activity; its activity is defined by its chemical functionality. It is used as a building block in organic synthesis. Its utility is demonstrated by its successful incorporation into PROTAC molecules, which then exhibit biological activity by degrading their target proteins. The in vitro "activity" is therefore in the context of a chemical reaction (e.g., peptide coupling) to form the final bioactive molecule. |
| ln Vivo |
As a chemical linker, Cbz-N-amido-PEG2-acid is not used in vivo. Its purpose is for the chemical synthesis of PROTACs or other conjugates. These final conjugates may be studied in vivo, but the linker itself is not the active pharmaceutical ingredient. Its primary application is in medicinal chemistry and drug discovery.
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| Enzyme Assay |
The "experimental protocol" for this compound is a chemical synthesis procedure. A typical protocol involves deprotecting the Cbz group by hydrogenolysis (e.g., using Pd/C and H2) to yield the free amine. The terminal carboxylic acid is then activated (e.g., with EDC or HATU) and reacted with a primary amine on another molecule to form an amide bond. This process is used to conjugate the PEG linker to other chemical moieties.
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| Cell Assay |
Cellular experiments would not be conducted with this linker directly. Instead, the final PROTAC molecule that is synthesized using this linker would be tested in cells. The linker's role is to enable the synthesis of these bioactive compounds. Its properties, such as length and solubility, contribute to the overall properties of the final PROTAC.
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| Animal Protocol |
Cbz-N-amido-PEG2-acid is not used in animal experiments. It is a chemical reagent for synthesis, not a drug candidate for in vivo testing. Only the final PROTAC molecules synthesized from it would be evaluated in animal models for their in vivo efficacy and pharmacokinetics.
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| ADME/Pharmacokinetics |
This compound is a small molecule with a molecular weight of 311.33 and a molecular formula of C15H21NO6. It is a PEG-based linker with a defined spacer length (PEG2). Its physicochemical properties, such as solubility and reactivity, are well-suited for use in organic synthesis. The Cbz protecting group makes it stable under various reaction conditions.
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| Toxicity/Toxicokinetics |
Cbz-N-amido-PEG2-acid is a chemical reagent and is not intended for therapeutic use. Its toxicity is not a primary concern, as it is handled in a laboratory setting with standard chemical safety precautions. Its safety is evaluated in the context of the final PROTAC molecules, not the linker itself.
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| References | |
| Additional Infomation |
Cbz-N-amido-PEG2-acid is a polyethylene glycol (PEG)-based PROTAC linker containing a Cbz-protected amine and a terminal carboxylic acid. It is a chemical building block used in the synthesis of PROTAC protein degraders. It is not a drug and has no direct biological activity. It is categorized as a research chemical for use in medicinal chemistry and drug discovery. The compound is also known as Cbz-NH-PEG2-C2-acid.
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| Molecular Formula |
C15H21NO6
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|---|---|
| Molecular Weight |
311.33034491539
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| Exact Mass |
311.136
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| CAS # |
1347750-76-8
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| Related CAS # |
1334177-88-6
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| PubChem CID |
86276404
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
0.6
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
22
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| Complexity |
317
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
XOMNIRHPTPYIMF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H21NO6/c17-14(18)6-8-20-10-11-21-9-7-16-15(19)22-12-13-4-2-1-3-5-13/h1-5H,6-12H2,(H,16,19)(H,17,18)
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| Chemical Name |
3-[2-[2-(phenylmethoxycarbonylamino)ethoxy]ethoxy]propanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2120 mL | 16.0601 mL | 32.1203 mL | |
| 5 mM | 0.6424 mL | 3.2120 mL | 6.4241 mL | |
| 10 mM | 0.3212 mL | 1.6060 mL | 3.2120 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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