| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| ln Vitro |
Pro-GA (0-180 μM; 0-6 days) inhibited the growth of human bladder cancer UMUC3, T24 and RT112 cells in a dose-dependent manner, with IC50 values of 73.9 μM, 57.0 μM and 58.5 μM, respectively [1]. Pro-GA (100 μM) significantly enhanced the antitumor effect of mitomycin C on RT112, UMUC3 and T24 human bladder cancer cells [1]. Pro-GA (50-100 μM; 1-5 days) inhibited the viability of human breast cancer MCF7 cells in a dose-dependent manner, inhibited cellular DNA synthesis and reduced the number of viable cells [2]. Pro-GA (50 μM; 72 hours) upregulated the expression of cyclin-dependent kinase inhibitors p21, p27 and p16 in human breast cancer MCF7 cells [2]. Pro-GA (75 μM; 72 hours) increased the level of mitochondrial reactive oxygen species in human breast cancer MCF7 cells [2]. Pro-GA (50-75 μM; 96 hours) can induce senescence in human breast cancer MCF7 cells[2].
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|---|---|
| ln Vivo |
pro-GA (25 mg/kg; intraperitoneal injection; twice a week for 4 weeks) significantly inhibited the growth of MCF7 breast cancer xenografts in immunodeficient mice [2].
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| Animal Protocol |
Animal/Disease Models:CB-17 severe combined immunodeficiency mice (female, 14 weeks old, MCF7 human breast cancer cells inoculated into mammary fat pads) [2]
Doses: 25 mg/kg Route of Administration: Intraperitoneal injection; twice a week; for 4 weeks Experimental Results: Compared with the control group, this product significantly inhibited the growth of MCF7 xenograft tumors. After 4 weeks of treatment, the relative volume and weight of the tumors were significantly reduced. No significant change in mouse body weight was observed during the treatment period, indicating no significant toxicity. |
| References |
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| Molecular Formula |
C12H19NO7
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|---|---|
| Molecular Weight |
289.28
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| CAS # |
2222067-12-9
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| Appearance |
Off-white to light yellow solid-liquid mixture
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| SMILES |
O=C(OC)CCCC(N[C@@H](C)C(OCOC(C)=O)=O)=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: 请将本产品存放在密封且受保护的环境中（例如氮气下），避免暴露在潮湿和光照下。 |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~172.84 mM; with sonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.64 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD saline (4°C, store for one week): Dissolve 2 g of SBE-β-CD powder in 10 mL of saline until completely dissolved and clear. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.64 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4569 mL | 17.2843 mL | 34.5686 mL | |
| 5 mM | 0.6914 mL | 3.4569 mL | 6.9137 mL | |
| 10 mM | 0.3457 mL | 1.7284 mL | 3.4569 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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