| ln Vitro |
CHI3L1-IN-3 (11 g of compound) exhibited enhanced stability with prolonged plasma half-lives of 2.0 h (mice) and 2.5 h (humans), prolonged microsomal half-lives of 2.2 h (mice) and 2.8 h (humans), and reduced clearance rates of 15 mL/min/mg (mice) and 19 mL/min/mg (humans) [1]. CHI3L1-IN-3 (50 μM, 72 h) showed low cytotoxicity to hBMEC and HepG2 cells and no cytotoxicity to NHA cells [1]. CHI3L1-IN-3 (5-25 μM, 72 h) dose-dependently reduced the cell viability of GBM globules [1]. CHI3L1-IN-3 (10 μM, 72 h) reduced globule weight by more than 50%, consistent with extensive cell death and loss of structural cohesion [1]. CHI3L1-IN-3 (10 μM, 24-96 h) inhibited GBM globule migration by up to 60% [1]. CHI3L1-IN-3 showed antiproliferative activity in glioblastoma cell lines, with an IC50 value of 2.19 μM in U251 MG cells and 5.06 μM in T98G cells [1]. CHI3L1-IN-3 (5-25 μM, 72 h) dose-dependently reduced pSTAT3 levels in GBM globules [1].
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|---|---|
| Cell Assay |
Cell viability assay [1]
Cell Types: GBM spheroids Tested Concentrations: 5, 10, 25 μM Incubation Duration: 72 hours Experimental Results: The viability of GBM spheroids decreased in a dose-dependent manner. Cell migration assay [1] Cell Types: GBM spheroids Tested Concentrations: 5, 10, 25 μM Incubation Duration: 24, 48, 72, 96 hours Experimental Results: Inhibited GBM spheroid migration by 60%. |
| References |
| CAS # |
3109469-27-1
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|---|---|
| Appearance |
Off-white to light yellow solid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~88.71 mM; with sonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.44 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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