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| 25mg |
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Purity: ≥98%
BAMB-4 (formerly known as ITPKA-IN-C14) is a novel and highly potent membrane-permeable inhibitor of inositol-1,4,5-trisphosphate-3-kinase A (ITPKA) with IC50 of 37 uM in ADP-Glo Assay. Ectopic expression of the neuron-specific ITPKA in lung cancer cells increases their metastatic potential because the protein exhibits two actin regulating activities; it bundles actin filaments and regulates inositol-1,4,5-trisphosphate (InsP3)-mediated calcium signals by phosphorylating InsP3. Thus, in order to inhibit the metastasis-promoting activity of ITPKA, both its actin bundling and its InsP3kinase activity has to be blocked. BAMB-4 showed an inhibitory effect. Noteworthy, in kinase screens no targets of BAMB-4 were detected, indicating that the compound does not belong to the typical kinase inhibitors.
| ln Vitro |
ITPKA is inhibited by BAMB-4 (0.3–40 μM), with an IC50 value of 37 μM[1]. InsP3 kinase activity is inhibited by BAMB-4 (40 μM) at an IC50 of 20 μM[1]. BAMB-4 has a high cellular uptake and high specificity at 100 μM over night[1]. Km is higher and V max is lower in BAMP-4 (0, 10, 20, and 40 μM) [1]. BAMP-4 (0–30 μM) is a mixed type inhibitor that affects both ATP and InsP3 binding. It affects the turnover of two substrates by binding to InsP3, which has a higher inhibition specificity[1].
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| References |
[1]. Dominik Schröder, et al. Identification of a new membrane-permeable inhibitor against inositol-1,4,5-trisphosphate-3-kinase A. Biochem Biophys Res Commun
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| Molecular Formula |
C15H12N2O2
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| Molecular Weight |
252.27
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| CAS # |
891025-25-5
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| Related CAS # |
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| SMILES |
C1(C=CC(=CC=1)C)C(NC1=NOC2C1=CC=CC=2)=O
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.91 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9640 mL | 19.8200 mL | 39.6401 mL | |
| 5 mM | 0.7928 mL | 3.9640 mL | 7.9280 mL | |
| 10 mM | 0.3964 mL | 1.9820 mL | 3.9640 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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