Size | Price | Stock | Qty |
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50mg |
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100mg |
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250mg |
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500mg |
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Other Sizes |
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Simvastatin ammonium salt is an active metabolite of simvastatin lactone mediated by CYP3A4/5 in the intestinal wall and liver, acting as an inhibitor of HMG-CoA reductase.
ln Vitro |
In hCM cells treated with benzyl alcohol sulfate, simvastatin acid (0.1–20 μM; 24 h) significantly decreased ROS generation by 8.9% to 43% [2]. hCM- was altered by simvastatin acid (0.1–20 μM; 24 h).
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Cell Assay |
Western Blot Analysis[2]
Cell Types: hCM and HEK293 (transfected with OATP3A1) Tested Concentrations: 0.1, 1, 10 and 20 μM Incubation Duration: 24 h Experimental Results: OATP3A1 expression was diminished in a dose-dependent manner by 1.5% to 90% in both hCM and OATP3A1-expressing cells. |
References |
[1]. Eduardo Filipe Oliveira, et al. HMG-CoA Reductase inhibitors: an updated review of patents of novel compounds and formulations (2011-2015). Expert Opin Ther Pat. 2016 Nov;26(11):1257-1272.
[2]. Atilano-Roque A, et al. Characterization of simvastatin acid uptake by organic anion transporting polypeptide 3A1 (OATP3A1) and influence of drug-drug interaction. Toxicol In Vitro. 2017 Dec;45(Pt 1):158-165. |
Molecular Formula |
C25H39O6-.H4N+
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Molecular Weight |
453.61202
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CAS # |
139893-43-9
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Related CAS # |
Simvastatin hydroxy acid sodium;101314-97-0;Simvastatin acid;121009-77-6;Simvastatin acid-d6 ammonium
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SMILES |
O=C([O-])CC(O)CC(O)CCC1C(C)C=CC2=CC(C)CC(OC(C(C)(C)CC)=O)C12.[NH4+]
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~11.11 mg/mL (~24.49 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.11 mg/mL (2.45 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 11.1 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.11 mg/mL (2.45 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 11.1 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.11 mg/mL (2.45 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.2045 mL | 11.0227 mL | 22.0454 mL | |
5 mM | 0.4409 mL | 2.2045 mL | 4.4091 mL | |
10 mM | 0.2205 mL | 1.1023 mL | 2.2045 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.