Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
In human HT-29 colon cancer cells that express PAR1 endogenously, protease-activated receptor-1, PAR-1 antagonist, activates protein kinase C isoenzymes alpha and epsilon. At the cellular level, HT-29 cell migration and matrix adhesion were induced by Protease-Activated Receptor-1, PAR-1 Agonist, and thrombin through a PKCepsilon-dependent mechanism. This was determined by the fact that the PKC inhibitors bisindolylmaleimide I and the PKCepsilon translocation inhibitory peptide EAVSLKPT, but not Gö 6976, inhibited the effects of PAR1-mediated processes.
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References |
[1]. Stefanie Gödecke, et al. Thrombin-induced ATP release from human umbilical vein endothelial cells. Am J Physiol Cell Physiol. 2012 Mar 15;302(6):C915-23.
[2]. Heider I, et al. PAR1-type thrombin receptor stimulates migration and matrix adhesion of human colon carcinoma cells by a PKCepsilon-dependent mechanism. Oncol Res. 2004;14(10):475-482. |
Molecular Formula |
C37H59F3N10O11
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Molecular Weight |
876.92
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Related CAS # |
Protease-Activated Receptor-1, PAR-1 Agonist;141136-85-8
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O :~6.67 mg/mL (~7.61 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (5.70 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
 (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.1404 mL | 5.7018 mL | 11.4035 mL | |
5 mM | 0.2281 mL | 1.1404 mL | 2.2807 mL | |
10 mM | 0.1140 mL | 0.5702 mL | 1.1404 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.