Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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25mg |
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Other Sizes |
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Purity: ≥98%
MB-10, also known as MitoBloCK-10, is a novel, potent and first-in-class small molecule modulator that can attenuate the activity of protein-associated motor (PAM) complex. It has the ability to prevent Tim44's (C-terminal domain) binding to both the precursor and Hsp70.
ln Vitro |
For substrate input, MB-10 (100 μM) is a protein input diluent [1]. Protein input substrate input diluent MB-10 (0-100 μM) is used [1]. Protein input substrate input diluent MB-10 (0-100 μM) is used [1].
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Cell Assay |
Cell viability assay [1]
Cell Types: Integrate the plasmid expressing Su9-Ura3-myc into the LEU2 locus of WT and tim23-2 strains. Tested Concentrations: 100μM. Incubation Duration: 30 minutes. Experimental Results: The WT strain expressing Su9-Ura3 failed to grow, while the tim23-2 mutant expressing Su9-Ura3 grew faster. The strains grew at similar rates when the medium was supplemented with uracil. Cell viability assay [1] Cell Types: HeLa cells. Tested Concentrations: 0-100 μM. Incubation Duration: 24 hrs (hours). Experimental Results: Inhibited HeLa cell viability, IC50 was 17.2 μM. |
References |
[1]. Non Miyata, et al. Adaptation of a Genetic Screen Reveals an Inhibitor for Mitochondrial Protein Import Component Tim44. J Biol Chem. 2017 Mar 31;292(13):5429-5442.
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Molecular Formula |
C12H8FN3O3S
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Molecular Weight |
293.273624420166
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Exact Mass |
293.03
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Elemental Analysis |
C, 49.15; H, 2.75; F, 6.48; N, 14.33; O, 16.37; S, 10.93
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CAS # |
394694-98-5
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Related CAS # |
394694-98-5
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Appearance |
Solid powder
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SMILES |
C1=CC(=CC(=C1)F)C(=O)N/N=C/C2=CC=C(S2)[N+](=O)[O-]
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InChi Key |
QDYQZMWFIYLMNX-VGOFMYFVSA-N
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InChi Code |
InChI=1S/C12H8FN3O3S/c13-9-3-1-2-8(6-9)12(17)15-14-7-10-4-5-11(20-10)16(18)19/h1-7H,(H,15,17)/b14-7+
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Chemical Name |
3-fluoro-N-[(E)-(5-nitrothiophen-2-yl)methylideneamino]benzamide
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Synonyms |
MitoBloCK 10; MitoBloCK-10; MB10; MB 10; MB-10
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: ~125 mg/mL (~426.2 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (7.09 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.4098 mL | 17.0491 mL | 34.0983 mL | |
5 mM | 0.6820 mL | 3.4098 mL | 6.8197 mL | |
10 mM | 0.3410 mL | 1.7049 mL | 3.4098 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
MB-10 inhibits Tim44 binding to the precursor and to Hsp70, but not other components of the PAM complex. J Biol Chem . 2017 Mar 31;292(13):5429-5442. J Biol Chem . 2017 Mar 31;292(13):5429-5442. td> |
MB-10 is a potential attenuator of protein import into mitochondria. J Biol Chem . 2017 Mar 31;292(13):5429-5442. td> |
MB-10 inhibits the import of substrates that use the TIM23 import pathway. J Biol Chem . 2017 Mar 31;292(13):5429-5442. td> |
MB-10 inhibition of import depends on specific chemical characteristics. J Biol Chem . 2017 Mar 31;292(13):5429-5442. td> |