Size | Price | Stock | Qty |
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100mg |
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Other Sizes |
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Ferulic acid methyl ester (Methyl ferulate) is a naturally occurring ferulic acid analog isolated from Stemona tuberosa with anti-inflammatory and antioxidant activity.
ln Vitro |
Following treatment for six, eighteen, and forty-eight hours, BMDMs do not exhibit any cytotoxic effects from methyl ferulate (25 μg/mL) [2]. Methyl ferulate (Methyl ferulate; 5, 10, 25 µg/mL) suppresses primary bone marrow-derived macrophages at 10 and 25 µg/mL and inhibits TNFα, IL6, IFNγ, but not IL10. NO generation in BMDM cells [2]. At 25 µg/mL, methyl ferulate suppresses the expression of COX-2 and blocks p-p38 and p-JNK[2].
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Cell Assay |
Cell viability assay [2]
Cell Types: primary bone marrow-derived macrophages (BMDM) Tested Concentrations: 25 µg/mL Incubation Duration: 6 hrs (hours), 18 hrs (hours), 48 hrs (hours) Experimental Results: No cytotoxic effect on BMDM. Western Blot Analysis[2] Cell Types: RAW 246.7 Cell Tested Concentrations: 10 µg/mL and 25 µg/mL Incubation Duration: 1 hour before LPS stimulation Experimental Results: Significant inhibition of COX-2 expression at 25 µg/mL concentration. |
References |
[1]. Sultana R. Ferulic acid ethyl ester as a potential therapy in neurodegenerative disorders. Biochim Biophys Acta. 2012 May;1822(5):748-52.
[2]. Phuong NT, et al. Anti-inflammatory activity of methyl ferulate isolated from Stemona tuberosa Lour. Asian Pac J Trop Med. 2014 Sep;7S1:S327-31. |
Molecular Formula |
C₁₁H₁₂O₄
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Molecular Weight |
208.21
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Exact Mass |
194.0579
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CAS # |
2309-07-1
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Related CAS # |
(E)-Ferulic acid methyl ester;22329-76-6
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SMILES |
O=C(O)/C=C/C1=CC=C(O)C(OC)=C1
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Chemical Name |
(E)-3-(4-hydroxy-3-methoxyphenyl)acrylic acid
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Synonyms |
Methyl ferulateConiferic acid trans-Ferulic acid NSC-2821 NSC2821NSC 2821Ferulic Acid
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~480.28 mM)
H2O : ~2 mg/mL (~9.61 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.01 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.01 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.01 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 4.8028 mL | 24.0142 mL | 48.0284 mL | |
5 mM | 0.9606 mL | 4.8028 mL | 9.6057 mL | |
10 mM | 0.4803 mL | 2.4014 mL | 4.8028 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.