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Purity: ≥98%
ETH2120 (also known as Sodium ionophore III) is a Na+ ionophore that is suitable for the assay of sodium activity in blood, plasma, serum. etc. The sodium ionophore ETH2120, but not protonophores, stimulated hydrogen-dependent caffeate reduction by 280%, indicating that caffeate reduction is coupled to the buildup of a membrane potential generated by primary Na(+) extrusion. Caffeate reduction was coupled to the synthesis of ATP, and again, ATP synthesis coupled to hydrogen-dependent caffeate reduction was strictly Na(+) dependent and abolished by ETH2120, but not by protonophores, indicating the involvement of a transmembrane Na(+) gradient in ATP synthesis. The ATPase inhibitor N,N'-dicyclohexylcarbodiimide (DCCD) abolished ATP synthesis, and at the same time, hydrogen-dependent caffeate reduction was inhibited. This inhibition could be relieved by ETH2120.
| ln Vitro |
In addition to stimulating caffeate reduction, preincubation of the cells with sodium ionophore III (Na+) entirely eliminated ATP production. When cells in a constant state of caffeate reduction were exposed to sodium ionophore III, the intracellular ATP level was rapidly depleted[1]. Cells cultured in lactate sulfate are resistant to the Na+ ionophore ETH2120[2]. One possible extraction agent for nuclear seizure treatment is sodium ionophore III ligand, which is a particularly efficient receptor for the Eu3+ and Am3+ cations[3].
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| References |
[1]. Imkamp F, et al. Chemiosmotic energy conservation with Na(+) as the coupling ion during hydrogen-dependent caffeate reduction by Acetobacterium woodii. J Bacteriol. 2002 Apr;184(7):1947-51.
[2]. Wang L, et al. The role of Rnf in ion gradient formation in Desulfovibrio alaskensis. PeerJ. 2016 Apr 14;4:e1919. [3]. Makrlík, E, et al. Sodium Ionophore III as Very Effective Receptor for Trivalent Europium and Americium.J Solution Chem (2016) 45: 463. |
| Molecular Formula |
C34H52N2O4
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| Molecular Weight |
552.80
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| CAS # |
81686-22-8
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| SMILES |
O=C(N(C1CCCCC1)C2CCCCC2)COC3=CC=CC=C3OCC(N(C4CCCCC4)C5CCCCC5)=O
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2 mg/mL (3.62 mM) in Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8090 mL | 9.0449 mL | 18.0897 mL | |
| 5 mM | 0.3618 mL | 1.8090 mL | 3.6179 mL | |
| 10 mM | 0.1809 mL | 0.9045 mL | 1.8090 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Stimulation of hydrogen-dependent caffeate reduction by sodium ionophores.J Bacteriol.2002 Apr;184(7):1947-51. |
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Inhibition of ATP synthesis coupled to hydrogen-dependent caffeate reduction by the sodium ionophore ETH2120.J Bacteriol.2002 Apr;184(7):1947-51. |
Inhibition of hydrogen-dependent caffeate reduction by the ATPase inhibitor DCCD and relief of DCCD inhibition by the sodium ionophore ETH2120.J Bacteriol.2002 Apr;184(7):1947-51. |
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