| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 1g |
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Purity: ≥98%
Cycloheximide, produced by S. griseus, is a glutarimide antibiotic and an inhibitor of protein synthesis in eukaryotes (but not prokaryotes) with IC50 of 5-
| Targets |
protein synthesis ( IC50 = 532.5 nM ); RNA synthesis ( IC50 = 2.88 μM )
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| ln Vitro |
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| ln Vivo |
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| Enzyme Assay |
Cycloheximide (also known as naramycin A) is an inhibitor of eukaryotic protein synthesis, with in vivo IC50 values for protein synthesis of 532.5 nM and RNA synthesis of 2880 nM, respectively.
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| Cell Assay |
GF-1 cell monolayers (4.0 mL, 105 cells/mL) grown on 60-mm Petri dishes are cultured for a minimum of 20 hours, followed by two PBS rinses. The cells are then treated with cycloheximide (CHX, 0.33 µg/mL) and BKA (20 µM) ANT inhibitor for 0–5 days. Lastly, the GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) is infected for 0–5 days after the initial step (dpi). Following the incubation period, every sample is taken out of the medium and given a PBS wash. After that, cells are incubated in 100 µL of staining solution for 10 to 15 minutes.
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| Animal Protocol |
Mice: In this experiment, male ICR mice that are around two months old are utilized. IP cycloheximide is given in concentrations of 0, 30, 60, or 120 mg/kg (saline controls). Injections of cycloheximide are given half an hour before training. Mice amnesia is commonly studied at a dose of 120 mg/kg. Observe that rats receive much lower amnestic Cycloheximide doses (1-3 mg/kg) than mice do, which is consistent with a comparable difference in the LD50s of mice and rats. As measured 30–60 minutes after injection, cycloheximide doses of 120–150 mg/kg cause about 95% inhibition of brain protein synthesis, while doses of 30 mg/kg cause about 80% inhibition.
Rats: Methoxyflurane anesthesia is used to perform unilateral carotid artery ligation on 7-day-old Sprague Dawley rat pups. The right common carotid artery is permanently ligated with 4-0 silk after a midline incision in the neck. Every animal received surgery for a maximum of five minutes. Rats are returned to their mother for recovery and feeding for two hours after surgery. The pups are then placed in an airtight chamber that is partially submerged in a temperature-controlled water bath to maintain a constant 36°C inside during the 100-minute hypoxic exposure period (8% O2, 92% N2). The rat pups in the HI with Cycloheximide treatment group receive an intraperitoneal injection of Cycloheximide at a dose of 0.6 mg/kg at0,6,12, or 24 hours of recovery, while the HI control group receives an equal volume of normal saline. Once the rat pups have been returned to their dam, they are sacrificed. The entire brain tissue is removed under deep intraperitoneal pentobarbital anesthesia (60 mg/kg) for triphenyl tetrazolium chloride (TTC) and flow cytometry at 48 and 72 hours after HI, respectively.
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| References |
| Molecular Formula |
C15H23NO4
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| Molecular Weight |
281.35
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| Exact Mass |
281.16
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| Elemental Analysis |
C, 64.04; H, 8.24; N, 4.98; O, 22.75
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| CAS # |
66-81-9
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| Related CAS # |
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| Appearance |
Solid powder
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| SMILES |
C[C@H]1C[C@@H](C(=O)[C@@H](C1)[C@@H](CC2CC(=O)NC(=O)C2)O)C
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| InChi Key |
YPHMISFOHDHNIV-FSZOTQKASA-N
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| InChi Code |
InChI=1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
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| Chemical Name |
4-[(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl]piperidine-2,6-dione
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.39 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.39 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5543 mL | 17.7715 mL | 35.5429 mL | |
| 5 mM | 0.7109 mL | 3.5543 mL | 7.1086 mL | |
| 10 mM | 0.3554 mL | 1.7771 mL | 3.5543 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Experimental strategy of GTI-seq using ribosome E-site translation inhibitors.Proc Natl Acad Sci U S A.2012 Sep 11;109(37):E2424-32. |
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Impact of uORF features on translational regulation.Proc Natl Acad Sci U S A.2012 Sep 11;109(37):E2424-32. |
Cross-species conservation of alternative TIS positions and identification of translated ncRNA.Proc Natl Acad Sci U S A.2012 Sep 11;109(37):E2424-32. |
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