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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Purity: ≥98%
Decription: AMBMP (also known as BML-284; BML284) is a novel, potent and cell-permeable Wnt signaling activator with anti-inflammatory activity. AMBMP induces TCF-dependent transcriptional activity(EC50 =700 nM).
ln Vitro |
The migration and invasion capacities of MNK45 and AGS cells are markedly enhanced by BML-284 (10 μM; 24 hours), while the migration and invasion capacities of cells blocked by pizotifen are partially restored [1]. When compared to the NC group, BML-284 (10 μM; 24 h) dramatically increased the expression of β-catenin. Additionally, as compared to the pizotifen treatment group, it partially counteracted the effects of pizotifen on the expression of N- and E-cadherin in MNK45 and AGS cells [1].
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ln Vivo |
In a study where Tg (myl7:EGFP) transgenic embryos at 5.5 hpf were placed into cells with 20 embryos on a plate, BML-284 (10 ng) and pyrimethanil (4 mg/L) together partially prevented the teratogenic phenotype and heart abnormalities produced by pyrimethanil[1].
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Cell Assay |
Western Blot Analysis[2]
Cell Types: Human gastric cancer cell lines MNK45 and AGS[1] Tested Concentrations: 10 µM Incubation Duration: 24 hrs (hours) Experimental Results: β-catenin expression was induced in MNK45 and AGS and E-cadherin and N-cadherin expressing cells were retained . |
References |
[1]. Liu J, et al. A small-molecule agonist of the Wnt signaling pathway. Angew Chem Int Ed Engl. 2005 Mar 18;44(13):1987-90.
[2]. Ying Jiang, et al. Pizotifen inhibits the proliferation and invasion of gastric cancer cells. Exp Ther Med. 2020 Feb;19(2):817-824. [3]. Yunlong Meng, et al. Exposure to pyrimethanil induces developmental toxicity and cardiotoxicity in zebrafish. Chemosphere. 2020 Sep;255:126889. |
Molecular Formula |
C19H19CLN4O3
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Molecular Weight |
350.37
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CAS # |
853220-52-7
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Related CAS # |
BML-284 hydrochloride;2095432-75-8
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SMILES |
NC1=NC(C2=CC=CC(OC)=C2)=CC(NCC3=CC=C(OCO4)C4=C3)=N1.[H]Cl
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Chemical Name |
C19H18N4O3
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Synonyms |
AMBMP BML284 BML 284 BML-284 Wnt Agonist
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~285.41 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.94 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.8541 mL | 14.2706 mL | 28.5413 mL | |
5 mM | 0.5708 mL | 2.8541 mL | 5.7083 mL | |
10 mM | 0.2854 mL | 1.4271 mL | 2.8541 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.