Size | Price | Stock | Qty |
---|---|---|---|
100mg |
|
||
Other Sizes |
|
ln Vitro |
Advice (This is our suggested protocol, which should be adjusted to suit your particular needs as it only offers guidance). Assay for serum acid phosphatase[1]: 1. Mix 50 μL of 4-methylumbelliferate phosphate (5.0 mM), 10 μL of 1.0 M pH 6.0 buffer, and 5.0 μL of serum enzyme into a solution to make 100 μL. 2. Include the following reagents (final concentrations): 45 mM EDTA, 6 mM EGTA, 4 mM 1,10-phenanthroline, 6 mM 8 quinolinol, 6 mM 8-hydroxy-5-quinolinesulfonic acid, 20 mM sodium tartrate, 15 mM sodium fluoride, and 3000 mM 2-mercaptoethanol. 3. To stop the reaction, add 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer (pH 10.5) to the reaction mixture and incubate it for 15 minutes at 37°C. 4. Using a fluorometer, measure the fluorescence after thoroughly mixing the sample.
|
---|---|
References |
[1]. Chambers JP, et al. Determination of serum acid phosphatase in Gaucher's disease using 4-methylumbelliferyl phosphate. Clin Chim Acta. 1977 Oct 1;80(1):67-77.
[2]. Watanabe F, et al. The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane. Clin Chim Acta. 1979 Feb 1;91(3):273-6. [3]. Hao Chen, et al. Autonomic Molecular Transport for Ultrasensitive Surface-Enhanced Infrared Absorption Spectroscopy. ACS Appl. Polym. Mater. 2020, 2, 9, 3929-3935 |
Molecular Formula |
C10H9O6P
|
---|---|
Molecular Weight |
256.14866
|
CAS # |
3368-04-5
|
Related CAS # |
4-Methylumbelliferyl phosphate disodium;22919-26-2
|
SMILES |
O=C1C=C(C)C2=CC=C(OP(O)(O)=O)C=C2O1
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
H2O : ~20.83 mg/mL (~81.32 mM)
|
---|---|
Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (39.04 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
 (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.9040 mL | 19.5198 mL | 39.0396 mL | |
5 mM | 0.7808 mL | 3.9040 mL | 7.8079 mL | |
10 mM | 0.3904 mL | 1.9520 mL | 3.9040 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.