Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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Purity: ≥98%
2-NBDG is a fluorescent analogue of glucose and an indicator used for measuring glucose uptake by bacteria and live mammalian cells and in tumor biopsies. The uptake of 2-NBDG is competitively inhibited by D-glucose, but not L-glucose or sucrose, in E. coli.
ln Vitro |
1. Creation of the 2-NBDG operational solution 1.1 Set up the solution in stock. Making a 1 mM stock solution requires 2.92 mL DDH2O and 1 milligram 2-NBDG. 1.2 Use premade corpse-free cell culture medium or PBS loading solution in place of the working solution. Kindly use the 2-NBDG working solution at the appropriate concentration based on the current circumstances. 2. Using cell staining 2.1 Membranes in suspension: Use centrifugation to gather the cells, then add PBS and wash five times. Adherent cells: Place islet digested cells in with the culture media removed. Place PBS in and centrifuge, then discard the supernatant. Repeatedly wash for five minutes each. 2.2 Add 1 mL of the 2-NBDG working solution; proceed with caution for a duration of 5 to 60 minutes. 2.3 Remove the supernatant and centrifuge at 400 g for 3–4 minutes at 4°C. 2.4 Weigh the cells twice, giving them five minutes each time, then add PBS. 2.5 Employ the cells for observation after resuspending them in 1 mL of serum-free bone marrow or PBS. When measuring the optical density (OD) of cells at 540/570 nm using an ELISA enzyme label, if a viability test is conducted. The drug's logarithmic concentration densitometer was plotted against the control's vitality to determine the IC50.
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References |
[1]. Yamada K, et al. A real-time method of imaging glucose uptake in single, living mammalian cells. Nat Protoc. 2007;2(3):753-62.
[2]. Katsuya Yamada, et al. A real-time method of imaging glucose uptake in single, living mammalian cells. Nat Protoc. 2007;2(3):753-62. [3]. Zou C, et al. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J Biochem Biophys Methods. 2005 Sep 30;64(3):207-15. [4]. Zou C, et al. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J Biochem Biophys Methods. 2005 Sep 30;64(3):207-15. |
Molecular Formula |
C12H14N4O8
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Molecular Weight |
342.26200
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CAS # |
186689-07-6
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SMILES |
O=C[C@H](NC1=CC=C([N+]([O-])=O)C2=NON=C21)[C@H]([C@@H]([C@@H](CO)O)O)O
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InChi Key |
QUTFFEUUGHUPQC-ILWYWAAHSA-N
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InChi Code |
InChI=1S/C12H14N4O8/c17-3-6(11(20)12(21)8(19)4-18)13-5-1-2-7(16(22)23)10-9(5)14-24-15-10/h1-3,6,8,11-13,18-21H,4H2/t6-,8+,11+,12+/m0/s1
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Chemical Name |
2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose
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Synonyms |
2-NBDG; 2NBDG; 2 NBDG
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O : ~5 mg/mL (~14.61 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 3.33 mg/mL (9.73 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
 (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9218 mL | 14.6088 mL | 29.2176 mL | |
5 mM | 0.5844 mL | 2.9218 mL | 5.8435 mL | |
10 mM | 0.2922 mL | 1.4609 mL | 2.9218 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.