Size | Price | Stock | Qty |
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100mg |
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Other Sizes |
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Purity: ≥98%
Ouabain (Ouabain Octahydrate; Acocantherine; G-Strophanthin), a cardiac steroid, is a novel, potent and selective Na+/K+, -ATPase inhibitor, which binds to α2 /α3 subunit with Ki of 41 nM/15 nM. Ouabain (100 nM) inhibits ATPase activities with 25%. Ouabain (0.1 μM-1.0 μM) inhibits the Na+ pump and increases stored Ca2+ in cultured rat astrocytes. High Ouabain affinity isoforms are confined to a reticular distribution within the PM that paralleled underlying endoplasmic or sarcoplasmic reticulum.
References |
J Biol Chem.1991 May 15;266(14):9327-31;Proc Natl Acad Sci U S A.1997 Mar 4;94(5):1800-5.
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Molecular Formula |
C29H44O12
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Molecular Weight |
584.66
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Exact Mass |
584.2833
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CAS # |
630-60-4
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SMILES |
O=C1OCC([C@H]2CC[C@]3(O)[C@]4([H])CCC5(O)CC(O[C@H]6[C@@H]([C@@H]([C@H]([C@H](C)O6)O)O)O)CC(O)[C@]5(CO)[C@@]4([H])[C@H](O)C[C@]23C)=C1
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InChi Key |
LPMXVESGRSUGHW-KAPJGFEOSA-N
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InChi Code |
InChI=1S/C29H44O12/c1-13-22(34)23(35)24(36)25(40-13)41-15-8-19(32)28(12-30)21-17(3-5-27(28,37)9-15)29(38)6-4-16(14-7-20(33)39-11-14)26(29,2)10-18(21)31/h7,13,15-19,21-25,30-32,34-38H,3-6,8-12H2,1-2H3/t13-,15?,16+,17+,18+,19?,21+,22-,23+,24+,25-,26+,27?,28+,29-/m0/s1
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Chemical Name |
3-(6-Deoxy-alpha-L-mannopyranosyloxy)-1,5,11a,14,19-pentahydroxycard-20(22)-enolide
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Synonyms |
Ouabain Acocantherin Strodival NSC 25485 NSC-25485 NSC25485
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.7104 mL | 8.5520 mL | 17.1040 mL | |
5 mM | 0.3421 mL | 1.7104 mL | 3.4208 mL | |
10 mM | 0.1710 mL | 0.8552 mL | 1.7104 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.